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Phase-fluorimetry study on dielectric relaxation of human serum albumin

机译:相荧光法研究人血清白蛋白介电弛豫

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摘要

The dielectric relaxation (DR) of human serum albumin (HSA) was studied by the method of phase-fluorometry, The protein environment of the single tryptophan in HSA shows a relatively low-speed DR of sub-ns characteristic time. This relaxation can be measured as a decaying red-shift of the time-resolved fluorescence emission spectra. The details of calculations of time-emission matrices (TEM) and comparison to the fluorescence data of the reference solution of N-acetyl-L-tryptophanamide (NATA) are also presented. (C) 2000 Elsevier Science B.V. All rights reserved. [References: 35]
机译:用相荧光法研究了人血清白蛋白(HSA)的介电弛豫(DR),HSA中单个色氨酸的蛋白质环境表现出亚ns特征时间的相对低速DR。可以将弛豫测量为时间分辨的荧光发射光谱的衰减红移。还介绍了计算时间发射矩阵(TEM)以及与N-乙酰基-L-色氨酸酰胺(NATA)参比溶液的荧光数据进行比较的详细信息。 (C)2000 Elsevier Science B.V.保留所有权利。 [参考:35]

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