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首页> 外文期刊>Journal of microbiology and biotechnology >Factors Influencing the Production of Water-soluble Endopolysaccharides and Exopolysaccharides from Lentinus lepideus and their Effects on Immune Cytokine Production
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Factors Influencing the Production of Water-soluble Endopolysaccharides and Exopolysaccharides from Lentinus lepideus and their Effects on Immune Cytokine Production

机译：香菇生产水溶性内多糖和胞外多糖的影响因素及其对免疫细胞因子的影响

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摘要

An efficient method to produce water-soluble polysaccharides from Lentinus lepideus is described. The productivity of both endopolysaccharides (PPS) and exopolysaccharides (EPS) was compared under various culture conditions. The effect of treating their own PPS and EPS on immune cytokine production was also studied in relation to culture factors. High yield production of EPS required moderate culture temperature (25oC) as well as long culture period (16-20 days). In contrast, PPS production required high culture temperature (30oC) and short culture period ( days). Most of the carbon sources did not affect polysaccharides and mycelial production except for sucrose. Immune cytokine levels in the EPS treatment varied among carbon sources or culture periods. PPS did not appear to affect much on the production of cytokines, regardless of the culturing factors, except for the culture period. These results suggest that the optimal culture conditions for L. lepideus vary according to culture purposes, and different culture conditions should be used for different targets including mycelial biomass, EPS, and PPS. Whereas the immunomodulating activitiy of EPS appeared to be affected by culture conditions in L. lepideus, that of PPS did not.
机译：描述了一种从香菇香菇生产水溶性多糖的有效方法。比较了各种培养条件下内多糖（PPS）和胞外多糖（EPS）的生产率。还研究了与培养因子相关的处理自己的PPS和EPS对免疫细胞因子产生的影响。 EPS的高产量生产需要适中的培养温度（25oC）和较长的培养时间（16-20天）。相反，PPS的生产需要较高的培养温度（30oC）和较短的培养时间（天）。除蔗糖外，大多数碳源均不影响多糖和菌丝体的产生。 EPS处理中的免疫细胞因子水平因碳源或培养时期而异。无论培养因素如何，除培养期外，PPS似乎对细胞因子的生产影响不大。这些结果表明，对于L.ideopideus的最佳培养条件会根据培养目的而变化，并且应针对不同的靶标（包括菌丝体生物量，EPS和PPS）使用不同的培养条件。 EPS的免疫调节活性似乎受到L. leideideus的培养条件的影响，而PPS则不受。

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