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首页> 外文期刊>Biophysical Chemistry: An International Journal Devoted to the Physical Chemistry of Biological Phenomena >Analytical ultracentrifugation and the characterization of chromatin structure.
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Analytical ultracentrifugation and the characterization of chromatin structure.

机译：分析超离心和染色质结构表征。

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摘要

This mini review consists of two parts. The first part will provide a brief overview of the theoretical aspects involved in the two kinds of experiments that can be conducted with the analytical ultracentrifuge (sedimentation velocity and sedimentation equilibrium) as they pertain to the study of chromatin. In the following sections, I describe the analytical ultracentrifuge experiments which, in my opinion, have contributed the most to our understanding of chromatin. Few other biophysical techniques, with the exception of X-ray scattering and diffraction, have contributed as extensively as the analytical ultracentrifuge to the characterization of so many different aspects of chromatin structure. In the course of his scientific career, Professor Henryk Eisenberg has made many important contributions to the theoretical aspects underlying ultracentrifuge analysis, especially in the analysis of solutions of polyelectrolytes and biological macromolecules [H. Eisenberg, Biological macromolecules and polyelectrolytes in solution, Clarendon Press, Oxford, 1976]. As an example he has devoted some of his research effort to the characterization of chromatin in solution. This review includes these important contributions.
机译：此迷你评论包括两个部分。第一部分将简要介绍分析超离心机可进行的两种与染色质相关的实验的理论方面（沉降速度和沉降平衡）。在以下各节中，我将介绍分析型超速离心实验，我认为这些实验对我们对染色质的理解贡献最大。除X射线散射和衍射外，很少有其他生物物理技术像分析超速离心机那样对染色质结构的许多不同方面的表征做出广泛贡献。在他的科学生涯中，亨利克·艾森伯格（Henryk Eisenberg）教授对超速离心分析的理论方面做出了许多重要贡献，特别是在聚电解质溶液和生物大分子溶液的分析方面[H. Eisenberg，溶液中的生物大分子和聚电解质，Clarendon Press，牛津，1976年]。例如，他致力于研究溶液中染色质的特性。这次审查包括这些重要的贡献。

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