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首页> 外文期刊>Journal of microbiology and biotechnology >Characterization of Mutations in AlHK1 Gene from Alternaria longipes: Implication of Limited Function of Two-Component Histidine Kinase on Conferring Dicarboximide Resistance
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Characterization of Mutations in AlHK1 Gene from Alternaria longipes: Implication of Limited Function of Two-Component Histidine Kinase on Conferring Dicarboximide Resistance

机译：长链格孢的AlHK1基因突变的表征：赋予组二甲酰亚胺抗性的二组分组氨酸激酶的有限功能的影响。

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摘要

Four series (S, M, R, and W) of Alternaria longipes isolates were obtained based on consecutive induction with Dimethachlon (Dim) and ultraviolet irradiation. These isolates were then characterized according to their tolerance to Dim, sensitivity to osmotic stress, and phenotypic properties. All the induced Dim-resistant isolates showed a higher osmosensitivity than the parental strains, and the last generation was more resistant than the first generation in the M, R, and W series. In addition, the changes in the Dim resistance and osmotic sensitivity were not found to be directly correlated, and no distinct morphologic characteristics were found among the resistant and sensitive isolates, with the exception of the resistant isolate K-11. Thus, to investigate the molecular basis of the fungicide resistance, a group III two-component histidine kinase (HK) gene, AlHK1, was cloned from nineteen A. longipes isolates. AlHK1p was found to be comprised of a six 92- amino-acid repeat domain (AARD), HK domain, and response regulator domain, similar to the Os-1p from Neurospora crassa. A comparison of the nucleotide sequences of the AlHK1 gene from the Dim-sensitive and -resistant isolates revealed that all the resistant isolates contained a single-point mutation in the AARD of AlHK1p, with the exception of isolate K-11, where the AlHK1p contained a deletion of 107 amino acids. Moreover, the AlHK1p mutations in the isolates of each respective series involved the same amino acid substitution at the same site, although the resistance levels differed significantly in each series. Therefore, these findings suggested that a mutation in the AARD of AlHK1p was not the sole factor responsible for A. longipes resistance to dicarboximide fungicides.
机译：基于用二甲双胍（Dim）连续诱导和紫外线照射获得了四个系列（S，M，R和W）的链格孢菌。然后根据其对Dim的耐受性，对渗透压的敏感性和表型特性对这些分离株进行表征。在M，R和W系列中，所有诱导的Dim抗性分离株均显示出比亲本菌株更高的渗透敏感性，并且最后一代比第一代菌株具有更高的耐药性。另外，除抗性分离株K-11外，没有发现Dim抗性的变化与渗透敏感性的变化直接相关，在抗性和敏感分离株中未发现明显的形态特征。因此，为了研究抗药性的分子基础，从十九个长双歧杆菌分离物中克隆了III族两组分组氨酸激酶（HK）基因AlHK1。发现AlHK1p由六个92个氨基酸的重复结构域（AARD），HK结构域和应答调节域组成，类似于来自神经孢霉的Os-1p。对暗淡敏感和抗性分离株的AlHK1基因核苷酸序列进行比较后发现，除K-11分离株（其中AlHK1p含有AlHK1p）外，所有抗性分离株均在AlHK1p的AARD中包含一个单点突变。删除107个氨基酸。此外，尽管每个系列的抗性水平明显不同，但每个系列的分离物中的AlHK1p突变在相同位点具有相同的氨基酸取代。因此，这些发现表明，AlHK1p的AARD中的突变不是导致长曲霉对双羧酰亚胺杀真菌剂产生抗性的唯一因素。

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