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首页> 外文期刊>Biophysical Chemistry: An International Journal Devoted to the Physical Chemistry of Biological Phenomena >Water self-diffusion behavior in yeast cells studied by pulsed field gradient NMR
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Water self-diffusion behavior in yeast cells studied by pulsed field gradient NMR

机译：脉冲场梯度NMR研究酵母细胞中水的自扩散行为

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摘要

The water self-diffusion behavior in yeast cell water suspension was investigated by pulsed field gradient NMR techniques. Three types of water were detected, which differ according to the self-diffusion coefficients: bulk water, extracellular and intracellular water. Intracellular and extracellular water self-diffusion was restricted; the sizes of restriction regions were approximately 3 and 15-20 mum, respectively. The smallest restriction size was determined as inner cell size. This size and also cell permeability varied with the growth phase of yeast cell. Cell size increased, but permeability decreased with increasing growth time. The values of cell permeabilities P-1(d) obtained from time dependence of water self-diffusion coefficient were in good agreement with the permeabilities obtained from the exchange rate constants P-1(eff). The values of P-1(eff) were 7X10(-6), 1.2X10(-6) and 1.6X10(-6) m/s, and P-1(d), were 6.3X10(-6), 8.4X10(-7), 1.5X10(-6) m/s for yeast cells incubated for 9 h (exponential growth phase), 24 h (end of exponential growth phase), and 48 h (stationary growth phase), respectively. (C) 2003 Elsevier Science B.V. All rights reserved. [References: 23]
机译：通过脉冲场梯度核磁共振技术研究了酵母细胞水悬浮液中水的自扩散行为。检测到三种类型的水，它们的自扩散系数不同：散装水，细胞外和细胞内水。细胞内和细胞外水的自我扩散受到限制;限制区的大小分别约为3和15-20μm。最小限制大小被确定为内部单元大小。该大小以及细胞渗透性随酵母细胞的生长阶段而变化。细胞大小增加，但通透性随生长时间的增加而降低。从水的自扩散系数的时间依赖性获得的细胞渗透率P-1（d）的值与从交换率常数P-1（eff）获得的渗透率非常一致。 P-1（eff）的值为7X10（-6），1.2X10（-6）和1.6X10（-6）m / s，P-1（d）的值为6.3X10（-6），8.4分别孵育9小时（指数生长期），24小时（指数生长期结束）和48小时（静止生长期）的酵母细胞的X10（-7），1.5X10（-6）m / s。 （C）2003 Elsevier Science B.V.保留所有权利。 [参考：23]

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