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首页> 外文期刊>Journal of microbiology and biotechnology >Evaluation of β-1,4-Endoglucanases Produced by Bacilli Isolated from Paper and Pulp Mill Effluents Irrigated Soils
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Evaluation of β-1,4-Endoglucanases Produced by Bacilli Isolated from Paper and Pulp Mill Effluents Irrigated Soils

机译：纸浆和纸浆厂废水灌溉土壤中分离的芽孢杆菌产生的β-1,4-内切葡聚糖酶的评价

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摘要

A total of 10 cellulase-producing bacteria were isolated from soil samples irrigated with paper and pulp mill effluents. The sequencing of 16S rRNA gene revealed that all isolates belonged to different species of genus Bacillus. Among the different isolates, B. subtilis IARI-SP-1 exhibited a high degree of β-l,4-endoglucanase (2.5 IU/ml), β-l，4-exoglucanase (0.8IU/ml)， and β-glucosidase (0.084 IU/ml) activity, followed by B. amyloliquefaciens IARI-SP-2. CMC was found to be the best carbon source for production of endo/exoglucanase and β-glucosidase. The β-l,4-endoglucanase gene was amplified from all isolates and their deduced amino acid sequences belonged to glycosyl hydrolase family 5. Among the domains of different isolates, the catalytic domains exhibited the highest homology of 93.7%, whereas the regions of signal, leader, linker, and carbohydrate-binding domain indicated low homology (73-74%). These variations in sequence homology are significant and could contribute to the structure and function of the enzyme.
机译：从用纸和纸浆厂废水灌溉的土壤样品中分离出总共10种产生纤维素酶的细菌。 16S rRNA基因的测序表明所有分离株都属于芽孢杆菌属。在不同的分离株中，枯草芽孢杆菌IARI-SP-1表现出高水平的β-1,4-内葡聚糖酶（2.5 IU / ml），β-1、4-外切葡聚糖酶（0.8IU / ml）和β-葡萄糖苷酶（0.084 IU / ml）活性，其次是解淀粉芽孢杆菌IARI-SP-2。发现CMC是生产内切/外切葡聚糖酶和β-葡萄糖苷酶的最佳碳源。从所有分离株中扩增出β-1，4-内切葡聚糖酶基因，其推导的氨基酸序列属于糖基水解酶家族5。在不同分离株的结构域中，催化结构域表现出最高的同源性，为93.7％，而信号区，前导，接头和碳水化合物结合结构域表明同源性低（73-74％）。序列同源性的这些变化是显着的，并且可能有助于酶的结构和功能。

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