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首页> 外文期刊>Biophysical Chemistry: An International Journal Devoted to the Physical Chemistry of Biological Phenomena >Size-dependent mobile surface charge model of cell electrophoresis
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Size-dependent mobile surface charge model of cell electrophoresis

机译：细胞电泳的尺寸依赖性移动表面电荷模型

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A model that accurately predicts the effects of cellular size and electric field strength on electrophoretic mobility has been developed. Previous models have predicted that electrophoretic mobility (EPM) is dependent only on cell surface charge, bath viscosity and ionic strength of the electrolyte. However, careful analysis of experimental data from the literature shows that these models do not accurately depict the relationship between chemically determined surface charge and observed mobility. We propose a new model that accounts for electrically driven redistribution of mobile surface charge islands, such as the recently proposed lipid raft structures. This model predicts electrophoretic mobility as a function of a new dimensionless quantity, A, that incorporates the cell radius, the electric field strength, and the average diameter of charged membrane complexes. (C) 2004 Published by Elsevier B.V.
机译：已开发出可精确预测细胞大小和电场强度对电泳迁移率影响的模型。先前的模型已经预测，电泳迁移率（EPM）仅取决于电池表面电荷，浴液粘度和电解质的离子强度。但是，对文献中实验数据的仔细分析表明，这些模型不能准确地描述化学确定的表面电荷与观察到的迁移率之间的关系。我们提出了一种新模型，该模型考虑了电驱动的移动表面电荷岛的重新分布，例如最近提出的脂质筏结构。该模型预测电泳迁移率是新的无量纲量A的函数，该量将细胞半径，电场强度和带电膜复合物的平均直径结合在一起。 （C）2004由Elsevier B.V.发布

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