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首页> 外文期刊>Journal of Avian Medicine and Surgery >Differences in protein fractions of avian plasma among three commercial electrophoresis systems.
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Differences in protein fractions of avian plasma among three commercial electrophoresis systems.

机译：三种商业电泳系统中禽血浆蛋白组分的差异。

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摘要

Previous studies have defined the presence of 6 protein fractions in plasma from many psittaciform species. Additionally, extensive reference intervals have been published for many of these species with the Beckman Paragon electrophoresis system, which had been commonly used in clinical laboratories to analyze the protein fractions of avian plasma. In mid-2009, Beckman discontinued the Paragon product line, leaving 2 primary alternative systems: Helena and Sebia. To compare electrophoresis results from the 3 commercial protein electrophoresis systems, specimens from 40 African grey parrots (Psittacus erithacus) were analyzed with the electrophoresis systems from Beckman, Helena, and Sebia. Marked differences in fraction migration were found between the Beckman/Helena and Sebia systems, which manifested as a large decrease in prealbumin and an increase in alpha1 globulins in the latter system. Both proportional and constant errors were observed among the fraction quantitation data of both the Helena and Sebia systems compared with the Beckman system. Based on Bland-Altman plot data and imprecision studies, the Helena system appears more similar with the Beckman system, although neither the Helena nor the Sebia systems are identical to the Beckman system. Because of the differences in electrophoresis methods, clinicians should be careful to consistently use particular clinical laboratories. For best application, reference intervals should be established based on both species and electrophoresis system.
机译：先前的研究已经定义了许多鹦鹉形物种的血浆中存在6种蛋白质组分。此外，已经使用Beckman Paragon电泳系统为许多这些物种发布了广泛的参考区间，该参考区间已在临床实验室中普遍用于分析禽血浆的蛋白质组分。在2009年中，贝克曼终止了Paragon产品线，剩下了两个主要的替代系统：海伦娜（Helena）和塞尔维亚（Sebia）。为了比较3种商业蛋白质电泳系统的电泳结果，使用Beckman，Helena和Sebia的电泳系统分析了40只非洲灰鹦鹉（Psittacus erithacus）的标本。在贝克曼/海伦娜和塞比亚系统之间发现了部分迁移的明显差异，这表现为前白蛋白的大量减少和后者系统中α1球蛋白的增加。与贝克曼系统相比，海伦娜和塞比亚系统的馏分定量数据中均观察到比例误差和常数误差。根据Bland-Altman图数据和不精确度研究，尽管Helena系统和Sebia系统都不与Beckman系统相同，但Helena系统与Beckman系统更相似。由于电泳方法的差异，临床医生应小心谨慎地始终使用特定的临床实验室。为了获得最佳应用，应基于种类和电泳系统建立参考间隔。

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