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首页> 外文期刊>Journal of magnetic resonance >Investigating magnetically aligned phospholipid bilayers with EPR spectroscopy at Q-band (35 GHz): optimization and comparison with X-band (9 GHz)
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Investigating magnetically aligned phospholipid bilayers with EPR spectroscopy at Q-band (35 GHz): optimization and comparison with X-band (9 GHz)

机译：使用Q波段（35 GHz）的EPR光谱研究磁性排列的磷脂双层：与X波段（9 GHz）的优化和比较

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This paper presents the improvement and advantages of investigating magnetically aligned phospholipid bilayers (bicelles) utilizing electron paramagnetic resonance (EPR) spectroscopy at a microwave frequency of 35 GHz (Q-band) and at a high magnetic field strength of 1.25 T when compared to weaker magnetic fields for X-band EPR studies. The nitroxide spin label 3beta-doxyl-5alpha-cholestane (cholestane or CLS) was inserted into the bicelles and utilized to demonstrate the effects of macroscopic bilayer alignment through the measurement of orientational dependent hyperfine splittings. The effects of different lanthanide ions with varying degree of magnetic susceptibility anisotropy were examined. The requirement of minimal amounts of the Tm3+ and Dy3+ lanthanide ions for well-aligned bicelles were examined for Q-band and compared with amounts required for X-band bicelle alignment studies. At a magnetic field of 1.25 T (when compared to 0.63 T at X-band), the perpendicular and parallel orientation were aligned with lower concentrations of Dy3+ andT(3+), respectively, and thereby eliminating/minimizing the unwanted effects associated with lanthanide-protein interactions. Thus, it is much easier to magnetically align phospholipid bilayers at Q-band when compared to X-band. (C) 2004 Elsevier Inc. All rights reserved.
机译：本文介绍了在35 GHz微波频率（Q波段）和1.25 T的高磁场强度下使用电子顺磁共振（EPR）光谱研究磁性排列的磷脂双层（bicelles）的改进和优点X波段EPR研究的磁场。将一氧化氮自旋标记3beta-doxyl-5alpha-胆甾烷（胆甾烷或CLS）插入双胞胎中，并通过测量方向依赖性超细裂隙来证明宏观双层排列的效果。研究了不同镧系离子对不同磁化率各向异性的影响。对于Q波段，检查了排列良好的Bicell的最低Tm3 +和Dy3 +镧系元素离子的要求，并将其与X波段双束线排列研究所需的数量进行了比较。在1.25 T的磁场（与X波段的0.63 T相比）下，垂直和平行取向分别与较低浓度的Dy3 +和T（3+）对齐，从而消除/最小化了与镧系元素相关的不良影响-蛋白质相互作用。因此，与X波段相比，将Q波段的磷脂双层进行磁对准要容易得多。 （C）2004 Elsevier Inc.保留所有权利。

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