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首页> 外文期刊>Journal of magnetic resonance >Cross-polarization schemes for peptide samples oriented in hydrated phospholipid bilayers
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Cross-polarization schemes for peptide samples oriented in hydrated phospholipid bilayers

机译：面向水合磷脂双层膜的肽样品的交叉极化方案

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摘要

Continuous-wave, ramped amplitude, and frequency modulated cross-polarization schemes (abbreviated as CWCP, RACP, and FMCP, respectively) are evaluated for static samples in anisotropic phases, such as peptides oriented in lipid environments. It is shown experimentally that both RACP and FMCP give rise to 20% higher polarized signal intensity in comparison to CWCP. The CP matching bandwidths for CWCP and RACP are about the same. Because of its adiabaticity, FMCP has a much broader CP matching bandwidth than CWCP and RACP. In addition, the ~(15)N RF amplitude used at the center of the FMCP matching profile is much lower than that of the CWCP and RACP matching profiles. A sample of [~(15)N]Leu_4 labeled gramicidin A oriented in lipid bilayers was used to demonstrate these experiments.
机译：针对各向异性相中的静态样品（例如在脂质环境中定向的肽），评估了连续波，斜波幅度和频率调制的交叉极化方案（分别缩写为CWCP，RACP和FMCP）。实验表明，与CWCP相比，RACP和FMCP均可产生高出20％的极化信号强度。 CWCP和RACP的CP匹配带宽大致相同。由于其绝热性，FMCP具有比CWCP和RACP宽得多的CP匹配带宽。此外，在FMCP匹配配置文件的中心使用的〜（15）N RF幅度远低于CWCP和RACP匹配配置文件的幅度。以脂质双层定向的[〜（15）N] Leu_4标记的短杆菌肽A的样品用于证明这些实验。

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