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首页> 外文期刊>Journal of magnetic resonance >Probing membrane topology by high-resolution H-1-C-13 heteronuclear dipolar solid-state NMR spectroscopy
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Probing membrane topology by high-resolution H-1-C-13 heteronuclear dipolar solid-state NMR spectroscopy

机译：通过高分辨率H-1-C-13异核双极固态NMR光谱探测膜拓扑

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摘要

Membrane topology changes introduced by the association of biologically pertinent molecules with membranes were analyzed utilizing the H-1-C-13 heteronuclear dipolar solid-state NMR spectroscopy technique (SAMMY) on magnetically aligned phospholipid bilayers (bicelles). The phospholipids H-1-C-13 dipolar coupling profiles lipid motions at the headgroup, glycerol backbone, and the acyl chain region. The transmembrane segment of phospholamban, the antimicrobial peptide (KIGAKI)(3) and cholesterol were incorporated into the bicelles, respectively. The lipids H-1-C-13 dipolar coupling profiles exhibit different shifts in the dipolar coupling contour positions upon the addition of these molecules, demonstrating a variety of interaction mechanisms exist between the biological molecules and the membranes. The membrane topology changes revealed by the SAMMY pulse sequence provide a complete screening method for analyzing how these biologically active molecules interact with the membrane. (c) 2005 Elsevier Inc. All rights reserved.
机译：利用H-1-C-13异核双极固态NMR光谱技术（SAMMY）在磁性排列的磷脂双层（bicelles）上分析了生物相关分子与膜的结合所引起的膜拓扑变化。磷脂H-1-C-13偶极偶联可分析脂质在头基，甘油主链和酰基链区域的运动。磷酸lamban的跨膜段，抗菌肽（KIGAKI）（3）和胆固醇分别被纳入到bicelles。加入这些分子后，脂质H-1-C-13的偶极耦合轮廓在偶极耦合轮廓位置表现出不同的位移，表明生物分子与膜之间存在多种相互作用机制。 SAMMY脉冲序列揭示的膜拓扑变化为分析这些生物活性分子如何与膜相互作用提供了一种完整的筛选方法。 （c）2005 Elsevier Inc.保留所有权利。

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