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首页> 外文期刊>Journal of magnetic resonance >DEER-Stitch: Combining three- and four-pulse DEER measurements for high sensitivity, deadtime free data
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DEER-Stitch: Combining three- and four-pulse DEER measurements for high sensitivity, deadtime free data

机译：DEER针距：结合三脉冲和四脉冲DEER测量以获取高灵敏度，无死区的数据

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摘要

Over approximately the last 15 years the electron paramagnetic resonance (EPR) technique of double electron electron resonance (DEER) has attracted considerable attention since it allows for the precise measurement of the dipole-dipole coupling between radicals and thus can lead to distance information between pairs of radicals separated by up to ca. 8 nm. The "deadtime free" 4-pulse DEER sequence is widely used but can suffer from poor sensitivity if the electron spin-echo decays too quickly to allow collection of a sufficiently long time trace. In this paper we present a method which takes advantage of the much greater sensitivity that the 3-pulse sequence offers over the 4-pulse sequence since the measured electron spin-echo intensity (for equal sequence lengths) is greater. By combining 3- and 4-pulse DEER time traces using a method coined DEER-Stitch (DEERS) accurate dipole-dipole coupling measurements can be made which combine the sensitivity of the 3-pulse DEER sequence with the deadtime free advantage of the 4-pulse DEER sequence. To develop the DEER-Stitch method three systems were measured: a semi-rigid bis-nitroxide labeled nanowire, the bis-nitroxide labeled protein CD55 with a distance between labels of almost 8 nm and a dimeric copper amine oxidase from Arthrobacter globiformis (AGAO).
机译：大约在过去的15年中，双电子电子共振（DEER）的电子顺磁共振（EPR）技术引起了相当大的关注，因为它可以精确地测量自由基之间的偶极-偶极耦合，因此可以导致两对之间的距离信息的自由基之间的间隔最大为。 8海里广泛使用“无死区时间”的4脉冲DEER序列，但是如果电子自旋回波衰减太快而无法收集足够长的时间轨迹，则灵敏度可能会很差。在本文中，我们提出了一种方法，该方法利用了3脉冲序列提供的灵敏度比4脉冲序列更高的灵敏度，因为所测量的电子自旋回波强度（对于相等的序列长度）更大。通过使用铸造DEER-Stitch（DEERS）方法组合3脉冲和4脉冲DEER时间轨迹，可以进行精确的偶极-偶极耦合测量，将3脉冲DEER序列的灵敏度与4的无死角优势结合在一起脉冲DEER序列。为了开发DEER-Stitch方法，对三个系统进行了测量：半刚性双硝基氧标记的纳米线，双硝基氧标记的蛋白质CD55（标记之间的距离接近8 nm）和球形节杆菌（AGAO）的二聚铜胺氧化酶。 。

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