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首页> 外文期刊>Biophysical Chemistry: An International Journal Devoted to the Physical Chemistry of Biological Phenomena >A COMPARISON OF THE SURFACE ACTIVITY OF THE FUNGAL HYDROPHOBIN SC3P WITH THOSE OF OTHER PROTEINS
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A COMPARISON OF THE SURFACE ACTIVITY OF THE FUNGAL HYDROPHOBIN SC3P WITH THOSE OF OTHER PROTEINS

机译：真菌疏水蛋白SC3P与其他蛋白质表面活性的比较

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摘要

The fungal hydrophobin SC3p, a protein secreted by Schizophyllum commune, has become known to form SDS-insoluble layers and to change the physico-chemical properties of an interface. In this study, the surface activity of SC3p was studied by determining the interfacial tensions gamma(lv) and gamma(sl) during adsorption of SC3p at both the liquid-vapour and the solid-liquid interface utilizing the in situ technique axisymmetric drop-shape analysis by profile. To this end, protein solution droplets were put on the solid fluoroethylene-propylene-Teflon. At the liquid-vapour interface, SC3p caused a large decrease of gamma(lv) from 72 to 43 mJ m(-2) at the concentration of 0.1 mg ml(-1). At the solid-liquid interface, gamma(sl) was slightly decreased, whereas the contact angle theta increased, indicating an increase in hydrophobicity of FEP-Teflon, which is unique among the proteins studied so far. Earlier findings indicated a decrease in hydrophobicity of Teflon upon adsorption of SC3p, but this was after a washing and drying step. In order to reconcile these findings with those of the present study, adsorption of SC3p to hydrophobic surfaces is suggested to occur in bilayers. The second layer is supposed to be less strongly adsorbed than the first layer and can be easily removed by washing. [References: 23]
机译：已知由Schizophyllum commune分泌的蛋白质疏水蛋白SC3p会形成SDS不溶层并改变界面的物理化学性质。在这项研究中，SC3p的表面活性是通过使用原位技术轴对称液滴形状确定SC3p在液体和固液界面上吸附过程中的界面张力gamma（lv）和gamma（sl）来研究的按配置文件分析。为此，将蛋白溶液液滴置于固体氟乙烯-丙烯-特氟隆上。在液-气界面处，SC3p导致浓度为0.1 mg ml（-1）的γ（lv）从72 mJ m（-2）大幅降低至43 mJ m（-2）。在固液界面处，γ（sl）略有降低，而接触角theta增大，表明FEP-Teflon的疏水性增加，这是迄今为止研究的蛋白质中唯一的。较早的发现表明，在吸附SC3p后，聚四氟乙烯的疏水性降低，但这是在洗涤和干燥步骤之后。为了使这些发现与本研究的发现相一致，建议将SC3p吸附到疏水表面上是双层的。假定第二层的吸附力不如第一层，并且很容易通过洗涤除去。 [参考：23]

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