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首页> 外文期刊>Journal of Medical Virology >Is hepatitis G/GB virus-C virus hepatotropic? Detection of hepatitis G/GB virus-C viral RNA in liver and serum.
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Is hepatitis G/GB virus-C virus hepatotropic? Detection of hepatitis G/GB virus-C viral RNA in liver and serum.

机译：肝炎G / GB丙型肝炎病毒有肝功能吗？检测肝和血清中的G / GB丙型肝炎病毒C型病毒RNA。

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The recently identified hepatitis G virus (HGV, also named GB virus-C, GBV-C) appears to have similarities to hepatitis C virus and other flaviviridae. To better understand its clinical significance and hepatotropism, we collected liver tissue and matched serum samples from 56 patients undergoing liver transplantation. HGV/GBV-C RNA was detected by reverse transcription-nested PCR, using primers from the relatively conserved 5' noncoding region of the genome to detect HGV/GBV-C RNA and the amount was semiquantitatively estimated by serial 10-fold endpoint dilution. The presence and amount of HCV RNA was estimated by the same methodology. Seventeen patients (30%) had HGV/GBV-C RNA detectable either in liver or in serum, including two of three with cryptogenic liver disease. Interestingly, 5 of 17 (29%) patients had HGV/GBV-C RNA in serum but not liver, even with repeated testing of hepatic RNA from different portions of the liver. Furthermore, the titer of HGV/GBV-C RNA was significantly lower in liver than in serum in most samples (mean log titer, 1.33 vs. 2.56, P < 0.05). In contrast, all 21 patients with HCV RNA in serum also had the virus detectable in liver. In five patients coinfected with HCV and HGV/GBV-C, the mean titer of HCV RNA in liver was higher than that in serum (log titer, 2.8 vs. 3.0, P > 0.05). Thus, our results suggest that HGV/GBV-C is probably not hepatotropic and may replicate predominantly in sites other than the liver. These findings brings into question the role of HGV in causing significant liver disease.
机译：最近鉴定出的丙型肝炎病毒（HGV，也称为GB病毒C，GBV-C）似乎与丙型肝炎病毒和其他黄病毒科相似。为了更好地了解其临床意义和肝移植性，我们从56例接受肝移植的患者中收集了肝脏组织并匹配了血清样本。 HGV / GBV-C RNA通过逆转录嵌套式PCR检测，使用来自基因组相对保守的5'非编码区的引物检测HGV / GBV-C RNA，并通过连续10倍终点稀释法半定量估算了该量。 HCV RNA的存在和量通过相同的方法估计。肝或血清中可检测到17例患者（30％）的HGV / GBV-C RNA，其中包括三例中的两名患有隐源性肝病。有趣的是，在17名患者中，有5名（29％）的血清中含有HGV / GBV-C RNA，但没有肝脏，即使从肝脏的不同部位反复检测了肝RNA。此外，在大多数样品中，肝脏中HGV / GBV-C RNA的滴度显着低于血清（平均对数滴度，1.33对2.56，P <0.05）。相比之下，所有21位血清中HCV RNA的患者在肝脏中也可检测到该病毒。在5例同时感染HCV和HGV / GBV-C的患者中，肝脏中HCV RNA的平均滴度高于血清（对数滴度，分别为2.8和3.0，P> 0.05）。因此，我们的结果表明，HGV / GBV-C可能不是肝细胞性的，可能主要在肝脏以外的部位复制。这些发现使人们怀疑HGV在引起重大肝脏疾病中的作用。

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