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首页> 外文期刊>Journal of Medical Virology >Molecular characterization of G11P(25) and G3P(3) human rotavirus strains associated with asymptomatic infection in South India.
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Molecular characterization of G11P(25) and G3P(3) human rotavirus strains associated with asymptomatic infection in South India.

机译：G11P（25）和G3P（3）人类轮状病毒毒株与无症状感染在南印度的分子表征。

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摘要

Rotaviruses are the major etiological agents of diarrhea in children less than 5 years of age. Two unusual rotavirus strains not previously reported in India, G11P[25] (CRI 10795) and G3P[3] (CRI 33594) were isolated from faecal samples of asymptomatic children in India. The strains were characterized by sequence analysis of the genes encoding the VP7, VP4, VP6, and NSP4. The G11P[25] strain was closely related to the human G11P[25] strains from Bangladesh (with 98% identity at the nucleotide [nt] level and the amino acid [aa] level for the VP7 gene and 96% identity at the nt and 98% at the aa level for the VP4 gene). The G3P[3] strain was found to be related to a G3P[3] strain isolated in Thailand (CMH222; 88% identity at the nt level and 97% at aa level for the VP7 gene and 84% identity at the nt level and 90% at the aa level for the VP4 gene). Phylogenetic analysis of the VP6 and the NSP4 genes revealed that the Vellore G11P[25] strain was of VP6 subgroup II and NSP4 genotype B. The G3P[3] strain was identified as NSP4 genotype C and the VP6 gene showed 97% identity at the deduced amino acid level with strain CMH222 (Thailand) strain but did not cluster with sequences of SGI, SGII, SGI+II or SG-nonIonII. Both strains had gene segments of animal rotavirus origin suggesting inter-species transmission of rotavirus, and in the case of G11P[25] possibly underwent reassortment subsequently with human strains resulting in an animal-human hybrid strain.
机译：轮状病毒是5岁以下儿童腹泻的主要病因。从印度无症状儿童的粪便样本中分离出了印度以前未报道的两种不寻常的轮状病毒株G11P [25]（CRI 10795）和G3P [3]（CRI 33594）。通过编码VP7，VP4，VP6和NSP4的基因的序列分析来表征菌株。 G11P [25]菌株与孟加拉国的人类G11P [25]菌株密切相关（VP7基因在核苷酸[nt]水平上具有98％的同一性，而在nt7氨基酸上具有96％的同一性VP4基因的氨基酸水平为98％）。发现G3P [3]菌株与泰国分离出的G3P [3]菌株有关（CMH222; VP7基因在nt一级的同源性为88％，aa一级为97％，在nt一级的同源性为84％， VP4基因的氨基酸水平为90％）。对VP6和NSP4基因进行系统进化分析表明，Vellore G11P [25]菌株属于VP6亚型II和NSP4基因型B。G3P[3]菌株被鉴定为NSP4基因型C，VP6基因在NSP4基因组中的同源性为97％推导了CMH222（泰国）菌株的氨基酸水平，但未与SGI，SGII，SGI + II或SG-nonI / nonII的序列聚类。两种菌株均具有动物轮状病毒起源的基因片段，表明轮状病毒是种间传播，在G11P [25]的情况下，可能随后与人类菌株进行了重配，从而形成了动物-人杂种菌株。

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