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首页> 外文期刊>Journal of Medical Virology >Evaluation of reverse transcription loop-mediated isothermal amplification assays for rapid diagnosis of pandemic influenza A/H1N1 2009 virus.
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Evaluation of reverse transcription loop-mediated isothermal amplification assays for rapid diagnosis of pandemic influenza A/H1N1 2009 virus.

机译：评估逆转录环介导的等温扩增测定法，用于快速诊断大流行性流感A / H1N1 2009病毒。

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摘要

Two genetic diagnosis systems using reverse transcription-loop-mediated isothermal amplification (RT-LAMP) technology were evaluated: one for detecting the HA gene of the pandemic influenza A/H1N1 2009 virus (H1pdm RT-LAMP) and the other for detecting the matrix gene of the influenza A virus (TypeA RT-LAMP). The competence of these two RT-LAMP assay kits for the diagnosis of the pandemic influenza A/H1N1 2009 virus was compared using real-time RT-PCR assays developed recently on viruses isolated and clinical specimens collected from patients with suspected infection. TypeA RT-LAMP and H1pdm RT-LAMP showed almost the same sensitivity as real-time RT-PCR for viruses isolated. The sensitivity and specificity of TypeA RT-LAMP and H1pdm RT-LAMP were 96.3% and 88.9%, respectively, for clinical specimens. Considering that the ability of the two RT-LAMP assay kits for detection of the pandemic influenza A/H1N1 2009 virus was comparable to that of the real-time RT-PCR assays, and that the assays were completed within 1 hr and did not require any expensive equipment, these two RT-LAMP assays are promising rapid diagnostic tests for the pandemic influenza A/H1N1 2009 virus at the hospital bedside.
机译：评估了两种使用逆转录环介导的等温扩增（RT-LAMP）技术的遗传诊断系统：一种用于检测大流行性流感A / H1N1 2009病毒的HA基因（H1pdm RT-LAMP），另一种用于检测基质甲型流感病毒的基因（TypeA RT-LAMP）。使用最近针对分离出的病毒和从疑似感染患者中收集的临床标本开发的实时RT-PCR分析方法，比较了这两种RT-LAMP分析试剂盒诊断大流行性流感A / H1N1 2009病毒的能力。 TypeA RT-LAMP和H1pdm RT-LAMP对分离出的病毒显示出与实时RT-PCR几乎相同的灵敏度。对于临床标本，TypeA RT-LAMP和H1pdm RT-LAMP的敏感性和特异性分别为96.3％和88.9％。考虑到两个RT-LAMP分析试剂盒检测大流行性流感A / H1N1 2009病毒的能力与实时RT-PCR分析的能力相当，并且该分析在1小时内完成，不需要在任何昂贵的设备上，这两种RT-LAMP测定法都有望在医院病床旁快速诊断出2009年大流行性A / H1N1流感病毒。

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