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首页> 外文期刊>Biophysical Chemistry: An International Journal Devoted to the Physical Chemistry of Biological Phenomena >Reaction-diffusion waves of actin filament polymerization/depolymerization in Dictyostelium pseudopodium extension and cell locomotion.
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Reaction-diffusion waves of actin filament polymerization/depolymerization in Dictyostelium pseudopodium extension and cell locomotion.

机译：肌动蛋白丝聚合/解聚在假单胞菌假po延伸和细胞运动中的反应扩散波。

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摘要

Cell surface movements and the intracellular spatial patterns and dynamics of actin filament (F-actin) were investigated in living and formalin-fixed cells of Dictyostelium discoideum by confocal microscopy. Excitation waves of F-actin assembly developed and propagated several micrometers at up to 26 microm/min in cells which had been intracellularly loaded with fluorescently labeled actin monomer. Wave propagation and extinction corresponded with the initiation and attenuation of pseudopodium extension and cell advance, respectively. The identification of chemical waves was supported by the ring, sphere, spiral and scroll wave patterns, which were observed in the extensions of fixed cells stained with phalloidin-rhodamine, and by the similar, asymmetrical [F-actin] distribution in wavefronts in living and fixed cells. These F-actin patterns and dynamics in Dictyostelium provide evidence for a new supramolecular state of actin, which propagates as a self-organized, reaction-diffusion wave of reversible F-actin assembly and affects pseudopodium extension. Actin's properties of oscillation and self-organization might also fundamentally determine the nature of the eukaryotic cell's reactions of adaptation, timing and signal response.
机译：通过共聚焦显微镜研究了盘基网柄菌的活细胞和福尔马林固定细胞中肌动蛋白丝（F-actin）的细胞表面运动以及细胞内空间格局和动力学。 F-肌动蛋白装配体的激发波在细胞内装载了荧光标记的肌动蛋白单体的细胞中以高达26微米/分钟的速度发展并传播了几微米。波的传播和消光分别对应假po延伸和细胞前进的开始和衰减。化学波的鉴定受到环，球，螺旋和涡旋波模式的支持，这些模式在用鬼笔环肽-若丹明染色的固定细胞的延伸中观察到，并且在生活中波前具有类似的不对称[F-肌动蛋白]分布和固定细胞。 Dctyostestelium中的这些F-肌动蛋白模式和动力学为肌动蛋白的新超分子状态提供了证据，肌动蛋白以可逆性F-肌动蛋白组装体的自组织，反应扩散波传播并影响假pseudo的延伸。肌动蛋白的振荡和自组织特性也可能从根本上决定了真核细胞适应，时间和信号反应的反应性质。

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