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首页> 外文期刊>Journal of nanoscience and nanotechnology >Electrospun Collagen/Poly(L-lactic acid-co-ε-caprolactone) Hybrid Nanofibrous Membranes Combining with Sandwich Construction Model for Cartilage Tissue Engineering
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Electrospun Collagen/Poly(L-lactic acid-co-ε-caprolactone) Hybrid Nanofibrous Membranes Combining with Sandwich Construction Model for Cartilage Tissue Engineering

机译：电纺胶原/聚（L-乳酸-ε-己内酯）杂化纳米纤维膜与三明治构建模型相结合用于软骨组织工程

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Electrospinning has recently received much attention, showing great potential as a novel scaffold fabrication method for cartilage tissue engineering. In this study, we developed a biodegradable hybrid nanofibrous membrane of collagen and poly(L-lactic acid-co-ε-caprolactone) (PLCL, 75:25) by electrospinning for cartilage tissue engineering. The structure and cell affinity of collagen/PLCL membranes were analyzed by scanning electron microscopy (SEM) and microscopy. The sandwiched cell-scaffold constructs were kept in culture for 1 week in vitro and then implanted subcutaneously into nude mice for 4, 8 and 12 weeks. Gross observation, histological and immuno-histological evaluation, glycosaminoglycan (GAG) analysis and Young's modulus measurements were performed at each post-implantation time-point. Electrospun collagen/PLCL nanofibrous membranes could mimic the natural ECM and have good cell affinity. All the cell-scaffold constructs showed cartilage-like morphology with a white, smooth and glistening appearance after 4, 8 and 12 weeks of implantation. The abundance of GAG containing cartilaginous matrix appeared to increase greatly with implantation time. Furthermore, well-distributed cartilage and nearly no empty areas were observed in constructs even at 12 weeks post-implantation. In addition, the mechanical properties of the engineered cartilage after 12 weeks of implantation could reach 83% of that of native rabbit auricular cartilage. These results indicate that collagen/PLCL nanofibrous membranes with the sandwich construction model may serve as a new approach for cartilage tissue engineering.
机译：最近，静电纺丝备受关注，显示出作为软骨组织工程的新型支架制造方法的巨大潜力。在这项研究中，我们通过电纺技术开发了一种可生物降解的胶原蛋白和聚（L-乳酸-co-ε-己内酯）（PLCL，75:25）的杂化纳米纤维膜，用于软骨组织工程。胶原/ PLCL膜的结构和细胞亲和力通过扫描电子显微镜（SEM）和显微镜进行分析。夹心的细胞支架构建体在体外培养1周，然后皮下植入裸鼠4、8和12周。在每个植入后的时间点进行肉眼观察，组织学和免疫组织学评估，糖胺聚糖（GAG）分析和杨氏模量测量。电纺胶原蛋白/ PLCL纳米纤维膜可模仿天然ECM，并具有良好的细胞亲和力。在植入4、8和12周后，所有的细胞支架构建体均显示出类似软骨的形态，具有白色，光滑和闪闪发光的外观。含GAG的软骨基质的丰度似乎随植入时间的增加而大大增加。此外，甚至在植入后12周，在构造物中也观察到软骨分布均匀并且几乎没有空区域。此外，植入12周后工程软骨的机械性能可能达到天然兔耳软骨的83％。这些结果表明，具有三明治结构模型的胶原蛋白/ PLCL纳米纤维膜可作为软骨组织工程的新方法。

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