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首页> 外文期刊>Biophysical Chemistry: An International Journal Devoted to the Physical Chemistry of Biological Phenomena >Analysis of absorption spectra of purple bacterial reaction centers in the near infrared region by higher order derivative spectroscopy
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Analysis of absorption spectra of purple bacterial reaction centers in the near infrared region by higher order derivative spectroscopy

机译:高阶导数光谱法分析近红外区紫色细菌反应中心的吸收光谱

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摘要

Reaction centers (RCs) of purple bacteria are uniquely suited objects to study the mechanisms of the photosynthetic conversion of light energy into chemical energy. A recently introduced method of higher order derivative spectroscopy [I.K. Mikhailyuk, H. Lokstein, A.P. Razjivin, A method of spectral subband decomposition by simultaneous fitting the initial spectrum and a set of its derivatives, J. Biochem. Biophys. Methods 63 (2005) 10-23] was used to analyze the NIR absorption spectra of RC preparations from Rhodobacter (R.) sphaeroides strain 2R and Blastochloris (B.) viridis strain KH, containing bacteriochlorophyll (BChl) a and b, respectively. Q(y) bands of individual RC porphyrin components (BChls and bacteriopheophytins, BPheo) were identified.
机译:紫色细菌的反应中心(RC)是研究光能从光能到化学能的光合作用转化机制的独特对象。最近介绍的高阶导数光谱法[I.K. Mikhailyuk,H.Lokstein,A.P.Razjivin,一种通过同时拟合初始光谱及其一组衍生物的光谱子带分解方法,J.Biochem。生物物理学。方法63(2005)10-23]被用于分析球形红景天菌株2R和草绿假单胞菌菌株BH的RC制剂的NIR吸收光谱,所述菌株分别含有细菌叶绿素(BChl)a和b。确定了单个RC卟啉成分(BChls和噬菌素,BPheo)的Q(y)带。

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