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首页> 外文期刊>Biophysical Chemistry: An International Journal Devoted to the Physical Chemistry of Biological Phenomena >Potential of cadmium sulphide nanorods as an optical microscopic probe to the folding state of cytochrome C.
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Potential of cadmium sulphide nanorods as an optical microscopic probe to the folding state of cytochrome C.

机译：硫化镉纳米棒作为光学显微镜探针对细胞色素C折叠状态的潜力。

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摘要

The folding behavior of cytochrome C (Cyt-C) conjugated with CdS nanorods (CdSnr) is amenable to monitoring by bright field microscopy, the porosity and percolating behavior of such protein conjugated nanoclusters depending on the folding history prior to the conjugation. The method has been used to predict the thermal melting behavior as well as guanidine hydrochloride induced unfolding of Cyt-C. Dynamic light scattering studies indicate that the size distribution of the nanoforms widens in presence of the protein. Furthermore, there is emergence of clusters with higher conductivity and altered zeta potential. Increase of second virial coefficient of CdS nanoforms in the presence of Cyt-C (obtained from static light scattering experiments) implies presence of protein coat over the hydrophobic nanosurface. The results are supported by morphological changes observed through scanning electron microscopy (SEM). Accordingly, the X-ray diffraction pattern shows a change of crystallographic orientations of CdSnr in presence of Cyt-C.
机译：与CdS纳米棒（CdSnr）缀合的细胞色素C（Cyt-C）的折叠行为适合通过明场显微镜进行监测，取决于缀合之前的折叠历史，此类蛋白质缀合的纳米簇的孔隙率和渗滤行为。该方法已用于预测热熔行为以及盐酸胍诱导的Cyt-C的展开。动态光散射研究表明，在蛋白质存在下，纳米形式的尺寸分布变宽。此外，出现了具有更高电导率和改变的ζ电势的簇。在Cyt-C存在下（从静态光散射实验获得），CdS纳米形式的第二病毒系数增加意味着在疏水性纳米表面上存在蛋白质涂层。通过扫描电子显微镜（SEM）观察到的形态变化支持了结果。因此，在Cyt-C存在下，X射线衍射图显示CdSnr的晶体学取向的变化。

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