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首页> 外文期刊>Journal of molecular microbiology and biotechnology: JMMB >Analysis of the Elements of Catabolite Repression in Clostridium acetobutylicum ATCC 824
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Analysis of the Elements of Catabolite Repression in Clostridium acetobutylicum ATCC 824

机译：丙酮丁醇梭菌ATCC 824中分解代谢物阻遏的元素分析

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摘要

The ptsH gene, encoding the phosphotransferase protein HPr, from Clostridium acetobutylicum ATCC 824 was identified from the genome sequence, cloned and shown to complement a ptsH mutant of Escherichia coli. The deduced protein sequence shares significant homology with HPr proteins from other low-GC gram-positive bacterial, although the highly conserved sequence surrounding the Ser-46 phosphorylation site is not well preserved in the clostridial protein. Nevertheless, the HPr was phosphorylate in an ATP-dependent manner in cell-free extracts of C. acetobutylicum. Furthermore, purified His-tagged HPr from Bacillus subtilis was also a substrate for the clostridial HPr kinase/phosphorylase. This phosphorylation reaction is a key step in the mechanism of carbon catabolite repression proposed to operate in B. subtilis and other low-GC gram-positive bacteria. Putative genes encoding the HPr kinase/phosphory-lase and the other element of this model, namely the catabolite control protein CcpA, were identified from the C. acetobutylicum genome sequence, suggesting that a similar mechanism of carbon catabolite repression many operate in this industrially important organism.
机译：从基因组序列中鉴定出来自丙酮丁醇梭菌ATCC 824的编码磷酸转移酶蛋白HPr的ptsH基因，将其克隆并显示出与大肠杆菌的ptsH突变体互补。推导的蛋白质序列与来自其他低GC革兰氏阳性细菌的HPr蛋白具有显着的同源性，尽管在梭菌蛋白中并未很好地保留Ser-46磷酸化位点周围的高度保守的序列。然而，HPr在乙酰丁酸梭菌的无细胞提取物中以ATP依赖性方式被磷酸化。此外，从枯草芽孢杆菌纯化的带有His标签的HPr也是梭菌HPr激酶/磷酸化酶的底物。该磷酸化反应是拟在枯草芽孢杆菌和其他低GC革兰氏阳性细菌中起作用的碳分解代谢物阻遏机制中的关键步骤。从丙酮丁醇梭菌基因组序列中鉴定出了编码HPr激酶/磷酸化酶和该模型其他元素的推定基因，即分解代谢物控制蛋白CcpA，这表明许多抑制碳分解代谢物的类似机制在这一工业上很重要生物。

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