Ciprofloxacin affects conformational equilibria of DNA gyrase A in thepresence of magnesium ions

Sissi C; Perdona E; Domenici E; Feriani A; Howells AJ; Maxwell A

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Ciprofloxacin affects conformational equilibria of DNA gyrase A in thepresence of magnesium ions

机译：在镁离子存在下，环丙沙星影响DNA促旋酶A的构象平衡

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The conformational equilibria of the A subunit of DNA gyrase (GyrA), of its 59 kDa N-terminal fragment (GyrA59) and of the quinolone-resistant Ser-Trp83 mutant (GyrATrp83), were investigated in the presence of mono- and divalent metal ions and ciprofloxacin, a clinically useful antibacterial quinolone. The stability of the proteins was estimated from temperature denaturation, monitoring unfolding with circular dichroism spectroscopy. Two transitions were observed in GyrA and GyrATrp83, which likely reflect unfolding of the N and C-terminal protein domains. Accordingly, one thermal transition is observed for GyrA59.

机译：在存在一价和二价金属的情况下，研究了DNA促旋酶（GyrA）的A亚基，其59 kDa N端片段（GyrA59）和对喹诺酮耐药的Ser-Trp83突变体（GyrATrp83）的构象平衡。离子和环丙沙星，一种临床上有用的抗菌喹诺酮。通过温度变性，用圆二色性光谱监测展开情况来估计蛋白质的稳定性。在GyrA和GyrATrp83中观察到两个过渡，这可能反映了N和C端蛋白质结构域的展开。因此，对于GyrA59观察到一个热转变。

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《Journal of Molecular Biology》 |2001年第1期|共9页
作者
Sissi C; Perdona E; Domenici E; Feriani A; Howells AJ; Maxwell A;
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正文语种 eng
中图分类分子生物学;
关键词
Dna-gyrase a; Quinolone; Magnesium; Circular dichroism; Affinity; Escherichia-coli; A-protein; Topoisomerase-ii; B-protein; Biological-activity; Binding; Enzyme; Quinolones; Mechanism; Complex;

机译：脱氧核糖核酸酶a;喹诺酮;镁;圆二色性;亲和力;大肠杆菌;A蛋白;拓扑异构酶ii;B蛋白;生物活性;结合;酶;喹诺酮;机理;复杂;

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专利

1. Ciprofloxacin affects conformational equilibria of DNA gyrase A in thepresence of magnesium ions [J] . Sissi C, Perdona E, Domenici E, Journal of Molecular Biology . 2001,第1期

机译：在镁离子存在下，环丙沙星影响DNA促旋酶A的构象平衡
2. Contribution of mutations in DNA gyrase and topoisomerase IV genes to ciprofloxacin resistance in Escherichia coli clinical isolates [J] . Bansal S., Tandon V. International journal of antimicrobial agents . 2011,第3期

机译：DNA促旋酶和拓扑异构酶IV基因突变对大肠杆菌临床分离株环丙沙星耐药性的贡献
3. Interaction of nalidixic acid and ciprofloxacin with wild type and mutated quinolone-resistance-determining region of DNA gyrase A [J] . Vashist J, Vishvanath, Kapoor R, Indian Journal of Biochemistry & Biophysics . 2009,第2期

机译：萘啶酸和环丙沙星与野生型和DNA促旋酶A突变的喹诺酮耐药决定区的相互作用
4. Peptide mimics of subunit B of DNA gyrase in interactions studies with coumarin drugs [C] . Saulo S.Garrido, Andreza C.Scatigno, Eduardo Maffud Cilli, European Peptide Symposium . 2002

机译：肽模拟DNA乙酸盐亚甲酸酶与香豆素药物相互作用研究的模拟
5. Low-resolution Structural Characterization of DNA-Bound Gyrase Enzymes [D] . Baker, Nicole Marie 2011

机译：DNA结合的促旋酶的低分辨率结构表征
6. DNA gyrase gyrA mutations in ciprofloxacin-resistant strains of Staphylococcus aureus: close similarity with quinolone resistance mutations in Escherichia coli. [O] . S Sreedharan, M Oram, B Jensen, 1990

机译：金黄色葡萄球菌对环丙沙星耐药的菌株中的DNA促旋酶gyrA突变：与大肠杆菌中的喹诺酮类耐药突变密切相似。
7. Identification of DNA gyrase Subunit a Mutations Associated with Ciprofloxacin Resistance in Staphylococcus aureus Isolated from Nasal Infection in Kurdistan-Iran [O] . Roya Darbani, Chiako Farshadfar, Somayeh Tavana, 2017

机译：鉴定DNA腺苷酸亚基与Kurdistan-Iran鼻腔感染中葡萄球菌中的葡萄球菌中抗蛋白酶抗性相关的突变

Ciprofloxacin affects conformational equilibria of DNA gyrase A in thepresence of magnesium ions

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