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首页> 外文期刊>Journal of Molecular Biology >A method for prediction of the locations of linker regions within large multifunctional proteins, and application to a type I polyketide synthase.
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A method for prediction of the locations of linker regions within large multifunctional proteins, and application to a type I polyketide synthase.

机译：一种预测大型多功能蛋白质中接头区域位置的方法，并将其应用于I型聚酮化合物合酶。

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Multifunctional proteins often appear to result from fusion of smaller proteins and in such cases typically can be separated into their ancestral components simply by cleaving the linker regions that separate the domains. Though possibly guided by sequence alignment, structural evidence, or light proteolysis, determination of the locations of linker regions remains empirical. We have developed an algorithm, named UMA, to predict the locations of linker regions in multifunctional proteins by quantification of the conservation of several properties within protein families, and the results agree well with structurally characterized proteins. This technique has been applied to a family of fungal type I iterative polyketide synthases (PKS), allowing prediction of the locations of all of the standard PKS domains, as well as two previously unidentified domains. Using these predictions, we report the cloning of the first fragment from the PKS norsolorinic acid synthase, responsible for biosynthesis of the first isolatable intermediate in aflatoxin production. The expression, light proteolysis and catalytic abilities of this acyl carrier protein-thioesterase didomain are discussed.
机译：多功能蛋白质通常看起来是由较小蛋白质的融合产生的，在这种情况下，通常可以简单地通过切割分开结构域的接头区域将其分离成祖先成分。尽管可能由序列比对，结构证据或轻蛋白水解指导，但确定接头区域的位置仍是经验性的。我们已经开发了一种名为UMA的算法，可以通过量化蛋白质家族中多个特性的保守性来预测多功能蛋白质中接头区域的位置，并且该结果与结构表征的蛋白质非常吻合。此技术已应用于一类真菌I型迭代聚酮化合物合酶（PKS），可预测所有标准PKS域以及两个以前未识别的域的位置。使用这些预测，我们报告了从PKS降鸟梨酸合酶的第一个片段的克隆，该酶负责黄曲霉毒素生产中第一个可分离中间体的生物合成。讨论了该酰基载体蛋白-硫酯酶双结构域的表达，光蛋白水解和催化能力。

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