MYOTUBE-SPECIFIC ACTIVITY OF THE HUMAN ALDOLASE A M-PROMOTER REQUIRES AN OVERLAPPING BINDING SITE FOR NF1 AND MEF2 FACTORS IN ADDITION TO A BINDING SITE (M1) FOR UNKNOWN PROTEINS

Salminen M.; Fiszman MY.; Demignon J.; Kahn A.; Daegelen D. Maire P.; Spitz F.

首页> 外文期刊>Journal of Molecular Biology >MYOTUBE-SPECIFIC ACTIVITY OF THE HUMAN ALDOLASE A M-PROMOTER REQUIRES AN OVERLAPPING BINDING SITE FOR NF1 AND MEF2 FACTORS IN ADDITION TO A BINDING SITE (M1) FOR UNKNOWN PROTEINS

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MYOTUBE-SPECIFIC ACTIVITY OF THE HUMAN ALDOLASE A M-PROMOTER REQUIRES AN OVERLAPPING BINDING SITE FOR NF1 AND MEF2 FACTORS IN ADDITION TO A BINDING SITE (M1) FOR UNKNOWN PROTEINS

机译：人类醛缩酶M启动子的肌管特异性活性需要未知蛋白的结合位点（M1）之外的NF1和MEF2因子的重叠位点

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The human aldolase A gene is expressed in several tissues through the use of three alternative promoters. The activity of one of the promoters, pM, is restricted to skeletal muscle. We reported previously that a proximal 280 bp pM fragment confers tissue-specific expression to a CAT reporter gene in transgenic mice. This small regulatory region directs expression to muscle composed mainly of fast-twitch fibers. Here we show that a minimal promoter fragment from base-pairs -164 to +45 is sufficient to highly active pM during myoblast differentiation in cell culture and demonstrate that two DNA elements play a major role in this activation. These elements consist of a binding site (M1) for unknown ubiquitous proteins and aa overlapping binding site for MEF2 and NF1 families of transcription factors. The NF1 factor constitute the main binding activity on the MEF2/NF1 site and, interestingly, some of the DNA-protein complexes that form with muscle nuclear extracts on the NF1 element differ from those that form with non-muscular extracts. (C) 1995 Academic Press Limited [References: 71]

机译：通过使用三个替代启动子，人类醛缩酶A基因在几种组织中表达。启动子之一pM的活性仅限于骨骼肌。我们以前报道过，近端280 bp pM片段可将组织特异性表达赋予转基因小鼠CAT报告基因。这个小的调节区域将表达引导至主要由快速抽动纤维组成的肌肉。在这里，我们显示了一个最小的启动子片段，从碱基对-164到+45足以在细胞培养中的成肌细胞分化过程中实现高活性的pM，并证明了两个DNA元素在这种激活中起主要作用。这些元件由未知泛在蛋白的结合位点（M1）和MEF2和NF1转录因子家族的重叠结合位点组成。 NF1因子构成了在MEF2 / NF1位点上的主要结合活性，有趣的是，与NF1元件上的肌肉核提取物形成的某些DNA-蛋白质复合物不同于那些与非肌肉提取物形成的DNA-蛋白质复合物。（C）1995 Academic Press Limited [参考号：71]

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来源
《Journal of Molecular Biology》 |1995年第1期|共15页
作者
Salminen M.; Fiszman MY.; Demignon J.; Kahn A.; Daegelen D. Maire P.; Spitz F.;
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正文语种 eng
中图分类分子生物学;
关键词
Fast-muscle-specific promoter; Mef2; Mef3; Myogenesis; Nf1; Muscle-specific expression; Helix-loop-helix; Heavy-chain gene; Nuclear factor-i; Troponin-c gene; A gene; Subunit gene; Cyclic amplification; Regulatory elements; Transgenic mice;

机译：快肌特异性启动子;Mef2;Mef3;肌发生;Nf1;肌肉特异性表达;螺旋-环-螺旋;重链基因;核因子-i;肌钙蛋白-c基因;A基因;亚基基因;循环扩增;调节元件;转基因小鼠;

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1. MYOTUBE-SPECIFIC ACTIVITY OF THE HUMAN ALDOLASE A M-PROMOTER REQUIRES AN OVERLAPPING BINDING SITE FOR NF1 AND MEF2 FACTORS IN ADDITION TO A BINDING SITE (M1) FOR UNKNOWN PROTEINS [J] . Salminen M., Fiszman MY., Demignon J., Journal of Molecular Biology . 1995,第1期

机译：人类醛缩酶M启动子的肌管特异性活性需要未知蛋白的结合位点（M1）之外的NF1和MEF2因子的重叠位点
2. Human C4b-Binding Protein Has Overlapping, But Not Identical, Binding Sites for C4b and Streptococcal M Proteins [J] . Anna M. Blom, Karin Bergg?rd, Joanna H. Webb, The journal of immunology . 2000,第10期

机译：人类C4b结合蛋白具有重叠但不是完全相同的C4b和链球菌M蛋白的结合位点
3. Prostaglandin E2-Mediated Activation of HIV-1 Long Terminal Repeat Transcription in Human T Cells Necessitates CCAAT/Enhancer Binding Protein (C/EBP) Binding Sites in Addition to Cooperative Interactions Between C/EBPβ and Cyclic Adenosine 5′-Monophosphate Response Element Binding Protein [J] . Nancy Dumais, Salim Bounou, Martin Olivier, The journal of immunology . 2002,第1期

机译：前列腺素E2介导的人类T细胞中HIV-1长末端重复转录的激活，除了C /EBPβ和环状腺苷5'-单磷酸盐反应元件结合蛋白之间的相互作用之外，还需要CCAAT /增强子结合蛋白（C / EBP）结合位点。
4. IDENTIFYING INTERACTION SITES IN 'RECALCITRANT' PROTEINS: PREDICTED PROTEIN AND RNA BINDING SITES IN REV PROTEINS OF HIV-1 AND EIAV AGREE WITH EXPERIMENTAL DATA [C] . MICHAEL TERRIBILINI, JAE-HYUNG LEE, CHANGHUI YAN, Pacific Symposium on Biocomputing 2006 . 2006

机译：鉴定“还原蛋白”中的相互作用位点：HIV-1和EIAV的反向蛋白中的预测蛋白和RNA结合位点均符合实验数据
5. Substrate binding site flexibility of small heat shock proteins and factors contributing to efficient chaperone activity. [D] . Jaya, Nomalie Naomi. 2009

机译：小型热激蛋白的底物结合位点柔性和有助于有效伴侣活性的因子。
6. Deformed protein binding sites and cofactor binding sites are required for the function of a small segment-specific regulatory element in Drosophila embryos. [O] . C Zeng, J Pinsonneault, G Gellon, 1994

机译：果蝇胚胎中小片段特异性调控元件的功能需要变形的蛋白质结合位点和辅因子结合位点。
7. Myocyte Enhancer Factor 2 (MEF2)-Binding Site Is Required forGLUT4 Gene Expression in Transgenic Mice [O] . Martin V. Thai, Suresh Guruswamy, Kim T. Cao, 1998

机译：肌细胞增强子因子2（MEF2） - 绕线位点是在转基因小鼠中的Formlut4基因表达
8. DNA Binding Drugs Targeting the Regulatory DNA Binding Site of the ETS Domain Family Transcription Factor Associated with Human Breast Cancer [R] . Wang, Y. 2001

机译：靶向与人乳腺癌相关的ETs结构域家族转录因子的调节DNa结合位点的DNa结合药物

MYOTUBE-SPECIFIC ACTIVITY OF THE HUMAN ALDOLASE A M-PROMOTER REQUIRES AN OVERLAPPING BINDING SITE FOR NF1 AND MEF2 FACTORS IN ADDITION TO A BINDING SITE (M1) FOR UNKNOWN PROTEINS

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