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首页> 外文期刊>Journal of Molecular Biology >THE GENE FOR NUCLEOSIDE DIPHOSPHATE KINASE FUNCTIONS AS A MUTATOR GENE IN ESCHERICHIA COLI
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THE GENE FOR NUCLEOSIDE DIPHOSPHATE KINASE FUNCTIONS AS A MUTATOR GENE IN ESCHERICHIA COLI

机译：大肠杆菌中的磷酸二氢核苷激酶功能基因

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Nucleoside diphosphate (NDP) kinase is a key enzyme in the control of cellular concentrations of nucleoside triphosphates, and has been shown to play important roles in various cellular activities such as developmental control, signal transduction and metastasis in eukaryotic systems. In this study, the gene for NDP kinase of Escherichia coli (ndk) was disrupted and surprisingly found to be dispensable without any discernible effects on cell growth or morphology. However, a mutator phenotype was found in ndk-disruption strains; frequencies of spontaneous mutations to rifampicin resistance and nalidixic acid resistant significantly increased. A higher frequency in reversion mutations was observed with use of an amber mutation in the kanamycin-resistance gene in an ndk-disruption strain. Imbalance in dNTP pools, in particular a significant increase of the dCTP content was observed, which is likely to result in the higher spontaneous mutation rates. These results suggest that NDP kinase, although not essential, plays an important role in the appropriate balance of intracellular dNTP pools to maintain a high DNA replication fidelity: Strains with ndk(-) pykA(-) pykF(-) as well as ndk(-) scs(-) were constructed without any discernible effect an cell growth, indicating that there is yet another enzyme(s) catalyzing nucleoside triphosphate synthesis, in addition to NDP kinase, pyruvate kinases and succinyl CoA synthetase. (C) 1995 Academic Press Limited [References: 35]
机译：核苷二磷酸（NDP）激酶是控制细胞中核苷三磷酸浓度的关键酶，并已显示出在各种细胞活动中的重要作用，例如发育控制，信号转导和真核系统转移。在这项研究中，大肠杆菌（ndk）NDP激酶的基因被破坏，令人惊讶地发现它是可有可无的，对细胞生长或形态没有任何明显的影响。但是，在ndk-破坏菌株中发现了突变型。自发突变对利福平和耐萘啶酸的突变频率显着增加。在ndk干扰菌株的卡那霉素抗性基因中使用琥珀色突变，观察到逆向突变的频率更高。 dNTP库不平衡，特别是dCTP含量显着增加，这很可能导致更高的自发突变率。这些结果表明，NDP激酶虽然不是必需的，但在细胞内dNTP池的适当平衡中发挥重要作用，以维持高DNA复制保真度：具有ndk（-）pykA（-）pykF（-）和ndk（构建了-）scs（-）而没有任何明显的细胞生长效果，表明除NDP激酶，丙酮酸激酶和琥珀酰CoA合成酶外，还有另一种催化三磷酸核苷的酶。 （C）1995 Academic Press Limited [参考：35]

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