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首页> 外文期刊>Journal of Molecular Biology >GENETIC BASIS OF BACTERIOPHAGE HK97 PROHEAD ASSEMBLY
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GENETIC BASIS OF BACTERIOPHAGE HK97 PROHEAD ASSEMBLY

机译：噬菌体HK97头大会的遗传基础。

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摘要

We report studies to determine which bacteriophage genes are required for assembly of phage HK97 proheads and what roles they play. We identify the gene encoding the major capsid protein of phage HK97 and report its DNA sequence, together with the DNA sequences of the two genes immediately upstream from it. When the capsid protein is expressed from a plasmid in the absence of other phage-encoded proteins, it assembles, with good efficiency and accuracy into prohead-like structures composed of the unprocessed 42 kDa capsid protein. No separately encoded scaffolding protein is required for this assembly If the 25 kDa product of the next gene upstream is co-expressed with the capsid protein, the prohead structures that are produced undergo the normal morphogenetic cleavage, which removes 102 amino acids from the N terminus of each subunit, leaving 31 kDa subunits. The 25 kDa protein is therefore probably a phage-encoded protease. The third gene, upstream from the protease gene, encodes the portal protein. Presence of the portal protein is not required for assembly of the capsid protein. Analysis of the phenotypes of four single amino acid-substitution mutants in the capsid-protein gene leads to several insights into the functions of the capsid protein and its interactions with the putative protease. [References: 29]
机译：我们报告的研究，以确定噬菌体HK97前额集结所需的噬菌体基因及其发挥的作用。我们鉴定出编码噬菌体HK97的主要衣壳蛋白的基因，并报告其DNA序列，以及紧接其上游的两个基因的DNA序列。当在没有其他噬菌体编码蛋白的情况下从质粒表达衣壳蛋白时，衣壳蛋白可以高效且准确地组装成未经处理的42 kDa衣壳蛋白组成的前突样结构。此组装不需要单独编码的支架蛋白如果上游的下一个基因的25 kDa产物与衣壳蛋白共表达，则产生的前额结构会经历正常的形态发生裂解，从N末端去除102个氨基酸每个亚基的残基，留下31kDa的亚基。因此，25 kDa蛋白可能是噬菌体编码的蛋白酶。蛋白酶基因上游的第三个基因编码门蛋白。壳体蛋白的组装不需要门户蛋白的存在。衣壳蛋白基因中的四个单个氨基酸替代突变体的表型分析导致衣壳蛋白的功能及其与推定的蛋白酶的相互作用的一些见解。 [参考：29]

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