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首页> 外文期刊>Journal of Molecular Biology >Transcriptional regulation by antitermination. Interaction of RNA with NusB protein and NusB/NusE protein complex of Escherichia coli.
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Transcriptional regulation by antitermination. Interaction of RNA with NusB protein and NusB/NusE protein complex of Escherichia coli.

机译：通过反终止进行转录调控。 RNA与大肠杆菌NusB蛋白和NusB / NusE蛋白复合物的相互作用。

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摘要

A recombinant heterodimeric NusB/NusE protein complex of Escherichia coli was expressed under the control of a synthetic mini operon. Surface plasmon resonance measurements showed that the heterodimer complex has substantially higher affinity for the boxA RNA sequence motif of the ribosomal RNA (rrn) operons of E.coli as compared to monomeric NusB protein. Single base exchanges in boxA RNA reduced the affinity of the protein complex up to 15-fold. The impact of base exchanges in the boxA RNA on the interaction with NusB protein was studied by (1)H,(15)N heterocorrelation NMR spectroscopy. Spectra obtained with modified RNA sequences were analysed by a novel generic algorithm. Replacement of bases in the terminal segments of the boxA RNA motif caused minor chemical shift changes as compared to base exchanges in the central part of the dodecameric boxA motif.
机译：大肠杆菌的重组异二聚体NusB / NusE蛋白复合物在合成的微型操纵子的控制下表达。表面等离子体共振测量表明，与单体NusB蛋白相比，异二聚体复合物对大肠杆菌核糖体RNA（rrn）操纵子的boxA RNA序列基序具有更高的亲和力。 boxA RNA中的单碱基交换将蛋白质复合物的亲和力降低了15倍。通过（1）H，（15）N异相关NMR光谱研究了boxA RNA中碱基交换对与NusB蛋白相互作用的影响。用新颖的通用算法分析用修饰的RNA序列获得的光谱。与十二聚体boxA基序中央部分的碱基交换相比，boxA RNA基序末端片段中碱基的替换引起较小的化学位移变化。

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