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首页> 外文期刊>Journal of Molecular Biology >Promoter suppression in cultured mammalian cells can be blocked by the chicken beta-globin chromatin insulator 5 ' HS4 and matrix/scaffold attachment regions
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Promoter suppression in cultured mammalian cells can be blocked by the chicken beta-globin chromatin insulator 5 ' HS4 and matrix/scaffold attachment regions

机译：鸡β-珠蛋白染色质绝缘子5'HS4和基质/支架附着区域可阻断培养的哺乳动物细胞中的启动子抑制

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摘要

Studies have indicated that two transcriptionally active units can repress one another when they lie adjacent in head-to-tail tandem on a chromosome. Repression of a downstream (3') unit by an upstream (5') unit is known as "transcriptional interference", whereas repression of a 5' unit by a 3' unit is termed "promoter suppression". These two processes can occur between head-to-tail tandem copies of a transgene, or between transgenes and adjacent chromosomal genes. Interference can be blocked by inserting a transcription terminator between adjacent units. Here, we report that "promoter suppression" could be blocked by the insulator 5' DNasel hypersensitive site 4, or matrix/scaffold attachment regions (MAR/SARs), when these elements were interposed between adjacent units. Because intergenic spacers of many repeated eukaryotic genes contain MAR/SARs and insulators, our observations suggest that these elements have the ability to segregate repeated genes into domains that act independently of one another. Our observations also suggest strategies to design transgenes that can act as autonomous units of expression.
机译：研究表明，当两个转录活性单位在一条染色体上从头到尾串联在一起时，它们可以相互抑制。上游（5'）单元对下游（3'）单元的抑制被称为“转录干扰”，而3'单元对5'单元的抑制被称为“启动子抑制”。这两个过程可以在转基因的头尾串联复制之间，或者在转基因和邻近的染色体基因之间发生。可以通过在相邻单元之间插入转录终止子来阻止干扰。在这里，我们报告说，当这些元件插入相邻单元之间时，“启动子抑制”可能会被绝缘子5'DNasel超敏位点4或矩阵/支架附着区域（MAR / SARs）阻止。由于许多重复的真核基因的基因间隔区均包含MAR / SAR和绝缘子，因此我们的观察结果表明，这些元件具有将重复的基因分隔为彼此独立起作用的域的能力。我们的观察结果还提出了设计可以充当表达自主单位的转基因的策略。

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