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首页> 外文期刊>Journal of Molecular Biology >Unusual molecular architecture of the Yersinia pestis cytotoxin YopM: A leucine-rich repeat protein with the shortest repeating unit
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Unusual molecular architecture of the Yersinia pestis cytotoxin YopM: A leucine-rich repeat protein with the shortest repeating unit

机译：鼠疫耶尔森氏菌细胞毒素YopM的异常分子结构：具有最短重复单元的富含亮氨酸的重复蛋白

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摘要

Many Gram-negative bacterial pathogens employ a contact-dependent (type III) secretion system to deliver effector proteins into the cytosol of animal or plant cells. Collectively, these effectors enable the bacteria to evade the immune response of the infected organism by modulating host-cell functions. YopM, a member of the leucine-rich repeat protein superfamily, is an effector produced by the bubonic plague bacterium, Yersinia pestis, that is essential for virulence. Here, we report crystal structures of YopM at 2.4 and 2.1 Angstrom resolution. Among all leucine-rich repeat family members whose atomic coordinates have been reported, the repeating unit of YopM has the least canonical secondary structure. In both crystals, four YopM monomers form a hollow cylinder with an inner diameter of 35 Angstrom. The domain that targets YopM for translocation into eukaryotic cells adopts a well-ordered, alpha -helical conformation that packs tightly against the proximal leucine-rich repeat module. A similar alpha -helical domain can be identified in virulence-associated leucine-rich repeat proteins produced by Salmonella typhimurium and Shigella flexneri, and in the conceptual translation products of several open reading frames in Y. pestis.
机译：许多革兰氏阴性细菌病原体采用接触依赖型（III型）分泌系统将效应蛋白输送到动物或植物细胞的细胞质中。这些效应器共同使细菌能够通过调节宿主细胞功能逃避被感染生物体的免疫反应。 YopM是富含亮氨酸的重复蛋白超家族的成员，是鼠疫鼠疫耶尔森菌产生的效应子，对毒力至关重要。在这里，我们报告了YopM在2.4和2.1埃分辨率下的晶体结构。在所有已报道其原子坐标的富含亮氨酸的重复家族成员中，YopM的重复单元具有最少的典型二级结构。在两个晶体中，四种YopM单体形成一个内圆柱，其内径为35埃。靶向YopM易位进入真核细胞的域采用了排列整齐的α螺旋构象，紧紧围绕着富含亮氨酸的重复模块。可以在鼠伤寒沙门氏菌和弗氏志贺氏菌产生的与毒力相关的富含亮氨酸的重复蛋白中以及在鼠疫耶尔森菌中几个开放阅读框的概念翻译产物中鉴定出相似的α-螺旋结构域。

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