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首页> 外文期刊>Journal of Molecular Biology >Surface map comparison: Studying function diversity of homologous proteins
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Surface map comparison: Studying function diversity of homologous proteins

机译：表面图比较：研究同源蛋白的功能多样性

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A simplified protein surface cartography approach has been developed to assist in the analysis of surface features in homologous families, and thus to predict conservation or divergence of protein functions and protein-protein interaction patterns. A spherical approximation of protein surface was used, with a focus on charged and hydrophobic residues. The resulting surface map allows for qualitative analysis and comparison of surfaces of proteins, but can also be used to define a simple numerical measure of map similarity between two or more proteins. The latter was shown to be useful for function based classifications within large protein families. Surface map analysis was tested on several test cases: haemoglobins, death domains and TRAF domains. It was shown that surface map comparison allows a better function prediction than general sequence analysis methods and can reproduce known examples of functional variation within a divergent group of proteins. in another example, we predict novel, unexpected sets of common functional properties for seemingly distant members of a large group of divergent proteins. The method was also shown to be robust enough to allow using protein models from comparative modelling instead of experimental structures. (C) 2001 Academic Press. [References: 59]
机译：已开发出一种简化的蛋白质表面制图方法来协助分析同源家族中的表面特征，从而预测蛋白质功能和蛋白质-蛋白质相互作用模式的保守性或差异性。使用蛋白质表面的球形近似，重点是带电荷和疏水残基。所得的表面图谱可用于蛋白质表面的定性分析和比较，但也可用于定义两个或多个蛋白质之间图谱相似性的简单数值度量。后者被证明可用于大蛋白家族中基于功能的分类。在几个测试案例上测试了表面图分析：血红蛋白，死亡域和TRAF域。结果表明，与一般序列分析方法相比，表面图谱比较可以提供更好的功能预测，并且可以重现已知不同蛋白质组中功能变异的实例。在另一个示例中，我们预测了一大批趋异蛋白质中看似遥远的成员的新颖，出乎意料的通用功能特性集。该方法还显示出足够的鲁棒性，可以使用比较模型中的蛋白质模型代替实验结构。 （C）2001学术出版社。 [参考：59]

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