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首页> 外文期刊>Journal of Molecular Biology >Crystal structure of the liganded SCP-2-like domain of human peroxisomal multifunctional enzyme type 2 at 1.75 A resolution
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Crystal structure of the liganded SCP-2-like domain of human peroxisomal multifunctional enzyme type 2 at 1.75 A resolution

机译：人类过氧化物酶体多功能酶2型的配体SCP-2-like结构域的晶体结构，分辨率为1.75 A

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摘要

beta -Oxidation of amino acyl coenzyme A (acyl-CoA) species in mammalian peroxisomes can occur via either multifunctional enzyme type 1 (MFE-1) or type 2 (MFE-2), both of which catalyze the hydration of trans-2-enoyl-CoA and the dehydrogenation of 3-hydroxyacyl-CoA, but with opposite chiral specificity. MFE-2 has a modular organization of three domains. The function of the C-terminal domain of the mammalian MFE-2, which shows similarity with sterol carrier protein type 2 (SCP-2), is unclear. Here, the structure of the SCP-2-like domain comprising amino acid residues 618-736 of human MFE-2 (d Deltah Delta SCP-2L) was solved at 1.75 Angstrom resolution in complex with Triton X-100, ari analog of a lipid molecule. This is the first reported structure of an MFE-2 domain. The d Deltah Delta SCP-2L has an alpha/beta -fold consisting of five beta -strands and five alpha -helices; the overall architecture resembles the rabbit and human SCP-2 structures. However, the structure of d Deltah Delta SCP-2L shows a hydrophobic tunnel that traverses the protein, which is occupied by an ordered Triton X-100 molecule. The tunnel is large enough to accommodate molecules such as straight-chain and branched-chain fatty acyl-CoAs and bile acid intermediates. Large empty apolar cavities are observed near the exit of the tunnel and between the helices C and D. In addition, the C-terminal peroxisomal targeting signal is ordered in the structure and solvent-exposed, which is not the case with unliganded rabbit SCP-2, supporting the hypothesis of a ligand-assisted targeting mechanism.
机译：哺乳动物过氧化物酶体中的氨基酰基辅酶A（酰基辅酶A）物种的β-氧化可通过多功能酶1型（MFE-1）或2型（MFE-2）发生，这两种酶均催化反式2-的水合烯酰辅酶A和3-羟酰基辅酶A的脱氢，但具有相反的手性特异性。 MFE-2具有三个域的模块化组织。尚不清楚哺乳动物MFE-2的C末端结构域的功能，该功能与2型固醇载体蛋白（SCP-2）相似。在此，以1.75埃分辨率分辨了包含人类MFE-2氨基酸残基618-736（d Deltah Delta SCP-2L）的SCP-2-like结构域的结构，该结构与Triton X-100（α的类似物）形成复合物。脂质分子。这是MFE-2域的第一个报告结构。 d Deltah Delta SCP-2L具有一个alpha / beta折叠，由五个beta链和五个alpha螺旋组成;总体结构类似于兔子和人类的SCP-2结构。然而，d Deltah Delta SCP-2L的结构显示了一个穿越蛋白质的疏水通道，该通道被有序的Triton X-100分子占据。该通道足够大，可以容纳分子，例如直链和支链脂肪酰基辅酶A和胆汁酸中间体。在隧道出口附近以及螺旋C和D之间观察到大的空的非极性空腔。此外，C末端过氧化物酶体靶向信号在结构中和溶剂暴露下是有序的，未配体兔SCP-不是这种情况2，支持配体辅助靶向机制的假设。

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