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首页> 外文期刊>Journal of Molecular Biology >The structures of crystalline complexes of human serum amyloid p component with its carbohydrate ligand, the cyclic pyruvate acetal of galactose.
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The structures of crystalline complexes of human serum amyloid p component with its carbohydrate ligand, the cyclic pyruvate acetal of galactose.

机译：人血清淀粉样蛋白p成分与其碳水化合物配体（半乳糖的环状丙酮酸缩醛）的晶体复合物的结构。

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摘要

Two monoclinic (P2(1)) crystal forms of human serum amyloid P component (SAP) in complex with the 4,6-pyruvate acetal of beta-D-galactose (MObetaDG) were prepared. Structure analysis by molecular replacement and refinement at 2.2A resolution revealed that crystal form 1 (a=95.76A, b=70.53A, c=103.41A, beta=96.80 degrees ) contained a pentamer in the asymmetric unit with a structure very similar to that of the published search model. The mode of ligand co-ordination was also similar except that four of the five subunits showed bound ligand with an additional H-bond between O1 of the galactose and the side-chain of Lys79. One sub-unit showed no bound ligand and a vacant calcium site close to a crystal contact. The 2.6A resolution structure of crystal form 2 (a=118.60A, b=109.10A, c=120.80A and beta=95.16 degrees ) showed ten sub-units in the asymmetric unit, all with two bound calcium ions and ligand. The most extensive protein-protein interactions between pentamers describe an AB face-to-face interaction involving 15 ion pairs that sandwiches five molecules of bound MObetaDG at the interface.
机译：制备了与β-D-半乳糖的4,6-丙酮酸缩醛（MObetaDG）复合的人类血清淀粉样蛋白P组分（SAP）的两种单斜晶（P2（1））晶体形式。通过以2.2A分辨率进行分子置换和精细化处理进行的结构分析显示，晶型1（a = 95.76A，b = 70.53A，c = 103.41A，beta = 96.80度）在不对称单元中包含五聚体，其结构与已发布的搜索模型。除了五个亚基中的四个显示出结合的配体外，半乳糖的O1和Lys79的侧链之间还具有额外的H键，配体配位的方式也相似。一个亚单位显示没有结合的配体和接近晶体接触的空钙位。晶型2的2.6A分辨率结构（a = 118.60A，b = 109.10A，c = 120.80A和beta = 95.16度）显示了不对称单元中的十个亚单元，全部带有两个结合的钙离子和配体。五聚体之间最广泛的蛋白质-蛋白质相互作用描述了AB面对面的相互作用，涉及15个离子对，在界面处夹有5个结合的MObetaDG分子。

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