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首页> 外文期刊>Journal of Molecular Biology >FUNCTIONAL BASE-PAIRING INTERACTION BETWEEN HIGHLY CONSERVED ELEMENTS OF U3 SMALL NUCLEOLAR RNA AND THE SMALL RIBOSOMAL SUBUNIT RNA
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FUNCTIONAL BASE-PAIRING INTERACTION BETWEEN HIGHLY CONSERVED ELEMENTS OF U3 SMALL NUCLEOLAR RNA AND THE SMALL RIBOSOMAL SUBUNIT RNA

机译：U3小分子核糖核酸和小核糖体亚单位RNA的高度保守元素之间的功能性碱基对相互作用

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摘要

The U3 nucleolar RNA has a remarkably wide phyletic distribution extending from the Eukarya to the Archaea. It functions in maturation of the small subunit (SSU) rRNA through a mechanism which is as yet unknown but which involves base-pairing with pre-rRNA. The most conserved part of U3 is within 30 nucleotides of the 5' end, but as yet no function for this domain has been proposed. Elements within this domain are complementary to highly conserved sequences in the SSU rRNA which, in the mature form, fold into a universally conserved pseudoknot. The nature of the complementarity suggests a novel mechanism for U3 function whereby U3 facilitates correct folding of the pseudoknot. Wide phylogenetic comparison provides compelling evidence in support of the interaction in that significant complementary changes have taken place, particularly in the archaeon Sulfolobus, which maintain the base-pairing. Base-substitution mutations in yeast U3 designed to disrupt the base-pairing indicate that the interaction is probably essential. These include cold-sensitivity mutations which exhibit phenotypes similar to U3-depletion, but without impairment of the AO processing step, which occurs within the 5' ETS. These phenotypes are consistent with the destabilization of SSU precursors and partial impairment of the processing steps Al, at the 5' ETS/18 S boundary, and A2, within the ITS1. (C) 1996 Academic Press Limited [References: 38]
机译：U3核仁RNA具有从Eukarya到Archaea的非常广泛的系统分布。它通过尚不清楚的机制参与小亚基（SSU）rRNA的成熟，但该机制涉及与pre-rRNA的碱基配对。 U3的最保守部分在5'端的30个核苷酸之内，但尚未提出该结构域的功能。该结构域内的元件与SSU rRNA中高度保守的序列互补，后者以成熟形式折叠成普遍保守的假结。互补性的本质暗示了U3功能的新颖机制，由此U3促进了假结的正确折叠。广泛的系统发育比较提供了令人信服的证据来支持这种相互作用，因为发生了显着的互补性变化，尤其是在古生的Sulfolobus中，该碱基保持了碱基配对。旨在破坏碱基配对的酵母U3中的碱基取代突变表明相互作用可能是必不可少的。这些包括冷敏感性突变，其表现出类似于U3耗竭的表型，但不损害在5'ETS内发生的AO处理步骤。这些表型与SSU前体的不稳定和在5'ETS / 18S边界处的加工步骤A1以及在ITS1内的A2的部分损伤相一致。 （C）1996 Academic Press Limited [参考号：38]

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