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tmRNA-induced release of messenger RNA from stalled ribosomes

机译：tmRNA诱导从停滞的核糖体释放信使RNA

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摘要

A ribosome stalled on a truncated mRNA in the eubacterial cell can be rescued by tmRNA via a process called trans-translation. We demonstrate here that release of truncated mRNAs from stalled ribosomes accelerates significantly already after trans-peptidation following tmRNA binding to the ribosome. However, rapid release of truncated mRNA requires EF-G-dependent translocation of peptidyl-tmRNA from the A to the P site of the ribosome. We show also that the rate of mRNA release before and after peptidyl-tmRNA translocation correlates well with the rate of dissociation of deacylated tRNA, indicating that mRNA is retained on the ribosome mainly through codon:anticodon interaction with tRNA. The rate of mRNA release is reduced for mRNAs with strong Shine-Dalgarno (SD)-like sequences in the vicinity of the truncation site as well as for mRNAs with long 3' extensions downstream from the P-site codon. The reduced rate of release in the former case was due to a persisting SD-anti SD interaction between mRNA and the ribosome. (c) 2005 Elsevier Ltd. All rights reserved.
机译：停在真细菌细胞中截短的mRNA上的核糖体可以通过tmRNA通过称为转译的过程进行拯救。我们在这里证明了从停顿的核糖体中释放截短的mRNA后，tmRNA与核糖体结合后的转肽作用已经大大加速了。但是，截短的mRNA的快速释放需要EF-G依赖的肽基tmRNA从核糖体的A到P位点易位。我们还表明，肽基-tmRNA易位之前和之后的mRNA释放速率与脱酰tRNA的解离速率密切相关，表明mRNA主要通过与tRNA的密码子：反义齿相互作用保留在核糖体上。对于在截短位点附近具有强Shine-Dalgarno（SD）样序列的mRNA以及在P位密码子下游具有3'延伸长的mRNA，mRNA的释放速率会降低。在前一种情况下，释放速率降低是由于mRNA和核糖体之间持续存在SD-抗SD相互作用。 （c）2005 Elsevier Ltd.保留所有权利。

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