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首页> 外文期刊>Journal of Molecular Biology >Structural insight into the dioxygenation of nitroarene compounds: the crystal structure of nitrobenzene dioxygenase.
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Structural insight into the dioxygenation of nitroarene compounds: the crystal structure of nitrobenzene dioxygenase.

机译：硝基芳烃化合物双加氧的结构见解：硝基苯双加氧酶的晶体结构。

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摘要

Nitroaromatic compounds are used extensively in many industrial processes and have been released into the environment where they are considered environmental pollutants. Nitroaromatic compounds, in general, are resistant to oxidative attack due to the electron-withdrawing nature of the nitro groups and the stability of the benzene ring. However, the bacterium Comamonas sp. strain JS765 can grow with nitrobenzene as a sole source of carbon, nitrogen and energy. Biodegradation is initiated by the nitrobenzene dioxygenase (NBDO) system. We have determined the structure of NBDO, which has a hetero-hexameric structure similar to that of several other Rieske non-heme iron dioxygenases. The catalytic subunit contains a Rieske iron-sulfur center and an active-site mononuclear iron atom. The structures of complexes with substrates nitrobenzene and 3-nitrotoluene reveal the structural basis for its activity with nitroarenes. The substrate pocket contains an asparagine residue that forms a hydrogen bond to the nitro-group of the substrate, and orients the substrate in relation to the active-site mononuclear iron atom, positioning the molecule for oxidation at the nitro-substituted carbon.
机译：硝基芳族化合物广泛用于许多工业过程中，并已释放到被认为是环境污染物的环境中。通常，由于硝基的吸电子特性和苯环的稳定性，硝基芳族化合物具有抗氧化侵蚀的能力。然而，细菌Comamonas sp。菌株JS765可以与硝基苯一起生长，作为唯一的碳，氮和能量来源。生物降解是由硝基苯双加氧酶（NBDO）系统引发的。我们确定了NBDO的结构，该结构具有与其他Rieske非血红素铁双加氧酶相似的异六聚体结构。催化亚基包含一个里斯克铁硫中心和一个活性位点单核铁原子。具有底物硝基苯和3-硝基甲苯的配合物的结构揭示了其与硝基芳烃的活性的结构基础。底物袋包含天冬酰胺残基，该残基形成与底物的硝基氢键，并使底物相对于活性位单核铁原子取向，从而将用于氧化的分子定位在硝基取代的碳上。

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