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首页> 外文期刊>Journal of Molecular Biology >A Time-resolved Investigation of Ribosomal Subunit Association.
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A Time-resolved Investigation of Ribosomal Subunit Association.

机译：时间解析的核糖体亚基协会的调查。

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摘要

The notion that the ribosome is dynamic has been supported by various biochemical techniques, as well as by differences observed in high-resolution structures of ribosomal complexes frozen in various functional states. Yet, the mechanisms and extent of rRNA dynamics are still largely unknown. We have used a novel, fast chemical-modification technique to provide time-resolved details of 16S rRNA structural changes that occur as bridges are formed between the ribosomal subunits as they associate. Association of different 16S rRNA regions was found to be a sequential, multi-step process involving conformational rearrangements within the 30S subunit. Our results suggest that key regions of 16S rRNA, necessary for decoding and tRNA A-site binding, are structurally altered in a time-dependent manner by association with the 50S ribosomal subunits.
机译：核糖体是动态的这一观点已得到各种生化技术的支持，以及在各种功能状态下冷冻的核糖体复合物的高分辨率结构中观察到的差异。然而，rRNA动力学的机制和范围仍然很大程度上未知。我们已经使用了一种新颖的，快速的化学修饰技术来提供16S rRNA结构变化的时间分辨细节，这种变化是由于在核糖体亚基之间缔合而在它们之间形成桥梁而发生的。发现不同的16S rRNA区域的关联是一个连续的多步骤过程，涉及30S亚基内的构象重排。我们的结果表明，通过与50S核糖体亚基缔合，以时间依赖的方式在结构上改变了16S rRNA的关键区域，这些区域是解码和tRNA A位点结合所必需的。

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