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首页> 外文期刊>Journal of Molecular Biology >EXPRESSION PATTERNS AND TRANSCRIPT PROCESSING OF FTT-1 AND FTT-2, TWO C-ELEGANS 14-3-3 HOMOLOGUES
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EXPRESSION PATTERNS AND TRANSCRIPT PROCESSING OF FTT-1 AND FTT-2, TWO C-ELEGANS 14-3-3 HOMOLOGUES

机译：两个C-线虫14-3-3的FTT-1和FTT-2的表达模式和转录加工

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A wide diversity of biological functions have been attributed to the highly conserved and ubiquitous 14-3-3 protein family. Yet how much of this diversity is inherent in the basic structure of 14-3-3 and how much is due to isoform specific functions is not yet fully understood. Here, two Caenorhabditis elegans 14-3-3 isoforms whose protein sequences are 90% similar were found to differ significantly in both their genomic structure and expression patterns. The two genes, ftt-1 (IV) (fourteen-three-three) and ftt-2 (X), differ in both the position and sequence of their introns, Since the various intron/exon boundaries respect neither functional nor structural protein motifs, the introns appear to be relatively recent evolutionary additions. ftt-1(IV) encodes three germline enhanced transcripts, two of which are related through the differential use of alternative poly(A) addition sites. RNA in situ hybridization studies reveal high levels of ftt-1 throughout the gonad with particularly high levels in the distal arm. In contrast, ftt-2 (X) encodes a single transcript which is expressed somatically. In embryos, high levels of ftt-1 transcripts appear to be maternally supplied, whereas ftt-2 is expressed as an early zygotic transcript whose expression pattern later localizes to the posterior region of post-proliferative embryos. These expression pattern differences between ftt-1 and ftt-2 suggest that these two 14-3-3 isoforms perform distinct biological roles within the worm. (C) 1997 Academic Press Limited. [References: 61]
机译：高度保守且普遍存在的14-3-3蛋白家族被认为具有广泛的生物学功能。然而，这种多样性的多少是14-3-3的基本结构所固有的，还有多少是由于同工型的特定功能所致。在这里，发现两个蛋白质相似度为90％的秀丽隐杆线虫14-3-3同工型在其基因组结构和表达模式上均存在显着差异。 ftt-1（IV）（十四至三十三）和ftt-2（X）这两个基因在其内含子的位置和序列上都不同，因为各种内含子/外显子边界既不考虑功能性蛋白质也不涉及结构性蛋白质基序，内含子似乎是相对较新的进化成员。 ftt-1（IV）编码三个种系增强的转录本，其中两个通过差异使用其他poly（A）添加位点而相关。 RNA原位杂交研究表明整个性腺中都有高水平的ftt-1，而远端臂中的水平特别高。相反，ftt-2（X）编码一个以体细胞表达的转录本。在胚胎中，似乎由母体提供了高水平的ftt-1转录本，而ftt-2被表达为早期合子转录本，其表达模式后来定位于增生后胚胎的后部区域。 ftt-1和ftt-2之间的这些表达方式差异表明，这两个14-3-3同工型在蠕虫中起着不同的生物学作用。 （C）1997 Academic Press Limited。 [参考：61]

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