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首页> 外文期刊>Journal of Molecular Biology >HOW ACCURATELY AND PRECISELY CAN RNA STRUCTURE BE DETERMINED BY NMR
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HOW ACCURATELY AND PRECISELY CAN RNA STRUCTURE BE DETERMINED BY NMR

机译：NMR如何准确准确地确定RNA结构

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摘要

The great diversity of RNA biological functions has led to widespread interest in RNA structure. Advances in synthetic and spectroscopic techniques have recently allowed the extension of NMR methods of structure determination to RNA structures of significant size and increased biological significance. However, it has not yet been established how accurately and precisely RNA structure can be determined by NMR. The extensive simulations presented here establish credible limits on accuracy and precision of NMR-derived RNA structures and provide quantitative calibrations to evaluate new structures. Synthetic sets of NMR constraints were generated from a crystallographically-derived ribozyme structure. The target structure was then redetermined using approximations and computational protocols derived from our experimental work. The results demonstrate that the structure of RNA molecules of at least 15,000 Da can be determined with precision and accuracy of 1 to 1.5 Angstrom, comparable to values obtained for proteins of similar size. Most encouragingly, it is shown that larger, globular and biologically more important RNA structures can be determined with significantly better accuracy and precision than smaller, elongated structures investigated until now. (C) 1997 Academic Press Limited. [References: 33]
机译：RNA生物学功能的多样性已经引起人们对RNA结构的广泛兴趣。合成和光谱技术的进步最近允许将结构测定的NMR方法扩展到具有显着大小和更高生物学意义的RNA结构。然而，尚未确定如何通过NMR确定RNA结构的准确度和精确度。此处提供的广泛模拟为NMR衍生的RNA结构的准确性和精密度建立了可靠的极限，并提供了定量校准以评估新结构。 NMR约束的合成集是从晶体学上得出的核酶结构生成的。然后使用从我们的实验工作中得出的近似值和计算协议重新确定目标结构。结果表明，至少15,000 Da的RNA分子结构可以以1至1.5埃的精度和准确度来确定，与相似大小的蛋白质获得的值相当。最令人鼓舞的是，已表明，与目前为止研究的较小的细长结构相比，可以以更大的准确性和精密度确定较大的球形和生物学上更重要的RNA结构。 （C）1997 Academic Press Limited。 [参考：33]

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