Interplay between metal binding and cis/trans isomerization in legume lectins: structural and thermodynamic study of P. angolensis lectin.

Garcia Pino A; Buts L; Wyns L; Loris R

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Interplay between metal binding and cis/trans isomerization in legume lectins: structural and thermodynamic study of P. angolensis lectin.

机译：豆科植物凝集素中金属结合与顺式/反式异构化之间的相互作用：安哥拉凝集素的结构和热力学研究。

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The interplay between metal binding, carbohydrate binding activity, stability and structure of the lectin from Pterocarpus angolensis was investigated. Removal of the metals leads to a more flexible form of the protein with significantly less conformational stability. Crystal structures of this metal-free form show significant structural rearrangements, although some structural features that allow the binding of sugars are retained. We propose that substitution of an asparagine residue at the start of the C-terminal beta-strand of the legume lectin monomer hinders the trans-isomerization of the cis-peptide bond upon demetallization and constitutes an intramolecular switch governing the isomer state of the non-proline bond and ultimately the lectin phenotype.

机译：研究了紫檀（Pterocarpus angolensis）的金属结合，碳水化合物结合活性，稳定性和凝集素结构之间的相互作用。去除金属会导致蛋白形式更加灵活，构象稳定性大大降低。这种无金属形式的晶体结构显示出显着的结构重排，尽管保留了一些允许糖结合的结构特征。我们建议，在豆类植物凝集素单体的C末端β链开始处替换天冬酰胺残基会阻碍顺肽键在脱金属后的反式异构化，并构成支配非-异构体状态的分子内开关。脯氨酸键和最终的凝集素表型。

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《Journal of Molecular Biology》 |2006年第1期|共15页
作者
Garcia Pino A; Buts L; Wyns L; Loris R;
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正文语种 eng
中图分类基础医学;
关键词
Lectins; binding; Metals; Assault by stabbing; 外源凝集素类; 金属;

机译：Lectins;binding;Metals;Assault by stabbing;外源凝集素类;金属;

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1. 硅胶固载光敏剂的合成及其在反式维生素D3光敏异构化为顺式维生素D3中的应用 [J] . 高云燕, 游长江, 陈金平, 浙江大学学报：A卷英文版 . 2003,第001期
2. Interplay between metal binding and cis/trans isomerization in legume lectins: structural and thermodynamic study of P. angolensis lectin. [J] . Garcia Pino A, Buts L, Wyns L, Journal of Molecular Biology . 2006,第1期

机译：豆科植物凝集素中金属结合与顺式/反式异构化之间的相互作用：安哥拉凝集素的结构和热力学研究。
3. Preparation, crystal structures and isomerization kinetics of cis- and trans-[Co(dtc)(2)(PHPh2)(2)](+): thermodynamically and kinetically stable cobalt(III)-P bonds through interplay of sigma-donicity, pi- acidity, and steric bulkiness [J] . Iwatsuki S., Suzuki T., Hasegawa A., Journal of the Chemical Society. Dalton Transactions . 2002,第18期

机译：顺式和反式[Co（dtc）（2）（PHPh2）（2）]（+）的制备，晶体结构和异构化动力学：通过σ相互作用，热力学和动力学上稳定的钴（III）-P键，酸度和空间体积
4. Preparation, crystal structures and isomerization kinetics of cis- and trans-[Co(dtc)_2(PHPh_2)_2]~+: thermodynamically and kinetically stable cobalt(III)-P bonds through interplay of #sigma#-donicity, #pi#-acidity, and steric bulkiness [J] . Satoshi Iwatsuki, Takayoshi Suzuki, Atsushi Hasegawa, Journal of the Chemical Society, Dalton Transactions. Inorganic Chemistry . 2002,第18期

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5. A QM/MM Molecular Dynamics Simulation Study of the Cis-Trans Isomerization Process of N-Methylacetamide [C] . Yasushige Yonezawa, Daron M. Standley, Hiromitsu Shimoyama Japanese peptide symposium . 2011

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6. Conformational preferences and structural characterization of prolyl cis/trans isomerization of carbohydrate-templated proline mimetics of some model peptides using computational methods. [D] . Teklebrhan, Robel Berhe. 2009

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7. Effect of substituent on the thermodynamics of D-glucopyranoside binding to concanavalin A pea (Pisum sativum) lectin and lentil (Lens culinaris) lectin. [O] . F P Schwarz, S Misquith, A Surolia 1996

机译：取代基对D-吡喃葡萄糖苷与伴刀豆球蛋白A豌豆（Pisum sativum）凝集素和小扁豆（Lens culinaris）凝集素结合的热力学的影响。
8. Structural basis for the recognition of complex-type biantennary oligosaccharides by Pterocarpus angolensis lectin. [O] . Buts, Lieven, Garcia-Pino, Abel, Imberty, Anne A, 2006

机译：檀香紫罗兰凝集素识别复杂型双触角寡糖的结构基础。

Interplay between metal binding and cis/trans isomerization in legume lectins: structural and thermodynamic study of P. angolensis lectin.

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