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首页> 外文期刊>Journal of Molecular Biology >DISTRIBUTION OF RESTRICTION ENZYME RECOGNITION SEQUENCES ON BROAD HOST RANGE PLASMID RP4 - MOLECULAR AND EVOLUTIONARY IMPLICATIONS
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DISTRIBUTION OF RESTRICTION ENZYME RECOGNITION SEQUENCES ON BROAD HOST RANGE PLASMID RP4 - MOLECULAR AND EVOLUTIONARY IMPLICATIONS

机译：限制性宿主间质RP4上限制性酶识别序列的分布-分子和进化含义

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摘要

IncP alpha plasmids, exemplified by RP4, are remarkable for their broad host range. They contain strikingly few cleavage sites for many commonly used type II restriction enzymes but an overabundance of sites for certain enzymes that target G + C-rich sequences. To identify factors responsible for these distributions, the recently compiled nucleotide sequence of RP4 was analysed to determine the frequency of tetra- and hexanucleotide motifs in the 49 kb plasmid backbone. This is defined as the sectors encoding basic plasmid functions. The overabundant restriction targets in RP4 are concentrated in the backbone and contain overlapping copies of CGGC/GCCG, identified as the most abundant tetranucleotide motif in the plasmid. Motif frequencies in the RP4 backbone are shown to be similar to those in Pseudomonas aeruginosa, a natural host of RP4, with the notable exception that a number of 6-bp palindromes are underrepresented in the plasmid. It is proposed that 6-bp palindromes were counterselected as type II restriction enzyme recognition sequences. Conjugative transfer of RP4 and R751 (IncP beta) is unusually sensitive to restriction compared to enterobacterial plasmids of the LncFII and IncI1 groups, implying that IncP plasmids experienced particularly strong selection for loss of restriction targets. Pseudomonas spp. of rRNA homology group I specify many type II restriction enzymes that target 6-bp palindromes and are candidates for the evolutionary hosts of IncP alpha plasmids. (C) 1996 Academic Press Limited [References: 50]
机译：以RP4为例的IncP alpha质粒因其广泛的宿主范围而引人注目。对于许多常用的II型限制酶而言，它们的切割位点非常少，但是某些靶向富含G + C序列的酶的位点过多。为了确定造成这些分布的因素，分析了最近编辑的RP4核苷酸序列，以确定49 kb质粒主链中四核苷酸和六核苷酸基序的频率。这被定义为编码基本质粒功能的区段。 RP4中过量的限制性酶切靶标集中在骨架上，并包含CGGC / GCCG的重叠拷贝，被确定为质粒中最丰富的四核苷酸基序。 RP4骨架中的母题频率显示与铜绿假单胞菌（RP4的天然宿主）中的母题频率相似，但值得注意的例外是质粒中的6bp回文数不足。建议将6 bp回文序列作为II型限制酶识别序列进行反选择。与LncFII和IncI1组的肠细菌质粒相比，RP4和R751（IncP beta）的结合转移对限制性酶异常敏感，这意味着IncP质粒对于限制性酶靶丢失的选择特别强。假单胞菌rRNA同源性I组的一员指定了许多针对6 bp回文的II型限制酶，它们是IncP alpha质粒的进化宿主的候选对象。 （C）1996 Academic Press Limited [参考：50]

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