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首页> 外文期刊>Journal of Molecular Biology >TRANSFER RNA GENE REDUNDANCY AND TRANSLATIONAL SELECTION IN SACCHAROMYCES CEREVISIAE
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TRANSFER RNA GENE REDUNDANCY AND TRANSLATIONAL SELECTION IN SACCHAROMYCES CEREVISIAE

机译：糖酵母中的转移RNA基因冗余和翻译选择。

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摘要

A total of 274 transfer RNA genes, representing the entire tRNA gene set of the yeast Saccharomyces cerevisiae, has been extracted from the whole genome sequence of this organism using a dedicated search algorithm (Pol3scan). All tRNA genes were assigned to 42 classes of distinct codon specificity. Accordingly, four deviations from previously proposed rules for third position wobble pairing in yeast, three G:U and one A:I codon-anticodon pairings, were found to be required to account for the reading of 61 coding triplets. The gene copy number for individual tRNA species, which ranges from one to 16, correlates well with both the frequency of codon occurrence in a sample of 1756 distinct protein coding sequences (r = 0.82) and the previously measured intracellular content of 21 tRNA species, A close link between tRNA gene redundancy and the overall amino acid composition of yeast proteins was also observed. Regression analysis values for individual protein coding sequences proved to be effective descriptions of the translational selective pressure operating on a particular gene. A significantly stronger co-adaptation between codon choice and tRNA gene copy number was observed in highly expressed genes. These observations strongly support the notion that intracellular tRNA levels in normally growing yeast cells are mainly determined by gene copy number, which, along with codon choice, is the key parameter acted upon by translational selection. (C) 1997 Academic Press Limited. [References: 25]
机译：使用专用搜索算法（Pol3scan）已从该生物的全基因组序列中提取了总计274个转移RNA基因，代表了酿酒酵母的整个tRNA基因集。所有的tRNA基因都被分配到42种不同的密码子特异性类别。因此，发现需要与先前提出的用于酵母中第三位置摆动配对的规则的四个偏差，三个G：U和一个A：I密码子-反密码子配对来解释61个编码三联体的读数。单个tRNA种类的基因拷贝数范围为1到16，与1756个不同蛋白质编码序列样本中的密码子出现频率（r = 0.82）以及先前测得的21种tRNA种类的细胞内含量都相关，还观察到了tRNA基因冗余与酵母蛋白质的总氨基酸组成之间的紧密联系。单个蛋白质编码序列的回归分析值被证明是对特定基因进行操作的翻译选择性压力的有效描述。在高表达的基因中观察到密码子选择和tRNA基因拷贝数之间明显更强的共适应。这些观察结果强烈支持这样的观点，即正常生长的酵母细胞中胞内tRNA水平主要由基因拷贝数决定，这与密码子选择一起是翻译选择所作用的关键参数。 （C）1997 Academic Press Limited。 [参考：25]

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