• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Molecular Biology >STABILITY AND STRUCTURE OF DNA OLIGONUCLEOTIDES CONTAINING NON-SPECIFIC BASE ANALOGUES
【24h】

STABILITY AND STRUCTURE OF DNA OLIGONUCLEOTIDES CONTAINING NON-SPECIFIC BASE ANALOGUES

机译：包含非特定碱基类似物的DNA寡核苷酸的稳定性和结构

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The nature of DNA containing the deoxyribosyl derivative of 5-nitroindole has been investigated. 5-Nitroindole has been shown to give good stability when present in duplexes opposite natural bases, with only slightly reduced melting temperatures. However, enhanced stability occurs when it is incorporated as an additional bulged base in duplexes. It also markedly enhances the stability of duplexes when it is present as a pendant base at either the 5' or 3'-ends of the two strands. The stabilisation is presumed to be due to enhanced stacking interactions for the nitroindole base. Oligomers containing a number of consecutive 5-nitroindole residues form stable, stacked secondary structures. An oligomer containing 21 such substitutions is presumed to exist as a hairpin structure. This was further investigated by circular dichroism melting experiments, which demonstrated that the single-stranded oligomer contains significant secondary structure in the region of the 5-nitroindole tract, which appears to contain a double-stranded stem. X-ray analysis of 5-nitroindole deoxyriboside provides some indication of how the mode of stacking observed in crystals of the nucleoside may also be responsible for stabilising secondary structures of oligonucleotides. (C) 1997 Academic Press Limited. [References: 31]
机译：已经研究了含有5-硝基吲哚的脱氧核糖基衍生物的DNA的性质。当在与天然碱相反的双链体中存在时，5-硝基吲哚显示出良好的稳定性，并且熔融温度仅略微降低。但是，当将其作为双凸体中的另一个凸出碱基合并时，稳定性会增强。当它作为悬垂碱基存在于两条链的5'或3'末端时，它也显着增强了双链体的稳定性。据推测，该稳定是由于硝基吲哚碱的增强的堆叠相互作用。包含多个连续的5-硝基吲哚残基的低聚物形成稳定的堆叠二级结构。推测含有21个这样的取代的低聚物以发夹结构存在。通过圆二色性熔融实验进一步研究了这一点，该实验表明单链低聚物在5-硝基吲哚区域中似乎含有双链茎，该区域具有明显的二级结构。对5-硝基吲哚脱氧核糖核苷的X射线分析提供了一些指示，表明在核苷晶体中观察到的堆积方式也可能负责稳定寡核苷酸的二级结构。 （C）1997 Academic Press Limited。 [参考：31]

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号