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首页> 外文期刊>Journal of Molecular Biology >LIPID LOCATION IN DEOXYCHOLATE-TREATED PURPLE MEMBRANE AT 2.6 ANGSTROM
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LIPID LOCATION IN DEOXYCHOLATE-TREATED PURPLE MEMBRANE AT 2.6 ANGSTROM

机译：脱氧胆酸盐处理的紫色膜脂位在2.6埃

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摘要

A high resolution projection at 2.6 Angstrom of deoxycholate-treated purple membrane using only images has been obtained with a 200 keV FEG microscope operated at liquid helium temperature. Examination of this high quality map has allowed the following conclusions to be made: Comparison between the internal structure of the trimers of the native and the deoxycholate-treated crystal forms shows that almost every detail of the structure at high resolution is identical. The cell dimension change from 62.4 Angstrom to 57.9 Angstrom is accompanied by a loss of about half the normal lipids and a 2 degrees anticlockwise rotation of the trimer as a rigid body. Three of the lipids per bacteriorhodopsin molecule remain in identical positions relative to the trimer. In addition, from the projection map together with a packing analysis using the atomic model for bacteriorhodopsin, space for three further lipids has been identified making a total of six lipids per bacteriorhodopsin molecule in this crystal form. Finally, the surprisingly small rotation of the trimer between the two crystal forms with completely different Van der Waals contacts suggest that the crystals are held together by strong, long-range electrostatic interactions. (C) 1995 Academic Press Limited [References: 31]
机译：用200 keV FEG显微镜在液氦温度下操作，仅使用图像即可获得在2.6埃脱氧胆酸盐处理的紫色膜上的高分辨率投影。对该高质量图谱的检查可以得出以下结论：天然三聚体和脱氧胆酸盐处理的晶型三聚体的内部结构之间的比较表明，高分辨率下该结构的几乎每个细节都是相同的。细胞尺寸从62.4埃变为57.9埃，伴随着大约一半正常脂质的损失以及三聚体作为刚性体的逆时针旋转2度。每个细菌视紫红质分子中的三个脂质相对于三聚体保持在相同位置。另外，从投影图以及使用细菌视紫红质的原子模型的装填分析一起，已经鉴定出另外三种脂质的空间，从而使每个细菌视紫红质分子以这种晶体形式共有六个脂质。最后，三聚体之间在范德华接触完全不同的情况下，三聚体的出乎意料的小旋转表明晶体通过强的，长距离的静电相互作用保持在一起。 （C）1995 Academic Press Limited [参考号：31]

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