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首页> 外文期刊>Journal of Molecular Biology >DIRECT LOCALIZATION OF THE TRNAS WITHIN THE ELONGATING RIBOSOME BY MEANS OF NEUTRON SCATTERING (PROTON-SPIN CONTRAST-VARIATION)
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DIRECT LOCALIZATION OF THE TRNAS WITHIN THE ELONGATING RIBOSOME BY MEANS OF NEUTRON SCATTERING (PROTON-SPIN CONTRAST-VARIATION)

机译：利用中子散射（质子自旋对比度变化）手段在隆重的核糖体中直接定位TRNAS

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A new technique for neutron scattering, the proton-spin contrast-variation, improves the signal-to-noise ratio more than one order of magnitude as compared to conventional techniques. The improved signal enables small RNA ligands within a large deuterated ribonucleic acid-protein complex to be measured. We used this technique to determine the positions of the two tRNAs within the elongating ribosome before and after translocation. Using a four-sphere model for each of the L-shaped tRNAs, unequivocal solutions were found for the localization of the mass centre of both tRNAs. The centre of gravity is located in the interface cavity separating the ribosomal subunits near the neck of the 30 S subunit. It moves during translocation by 12(+/-4) Angstrom towards the head of the 30 S subunit and slightly towards the L1 protuberance of the 50 S subunit. (C) 1997 Academic Press Limited. [References: 39]
机译：质子自旋对比度变化是中子散射的新技术，与传统技术相比，其信噪比提高了一个数量级以上。改善的信号使得能够测量大氘核糖核酸-蛋白质复合物中的小RNA配体。我们使用这种技术来确定易位前后两个tRNA在核糖体中的位置。使用每个L形tRNA的四球模型，可以确定两种tRNA质心的定位。重心位于界面空腔中，该界面空腔将核糖体亚基分隔在30 S亚基的颈部附近。它在易位过程中向30 S亚基的头部移动12（+/- 4）埃，向50 S亚基的L1突起稍微移动。 （C）1997 Academic Press Limited。 [参考：39]

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