• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Molecular Biology >MAPPING THE PROTEIN SURFACE OF HUMAN IMMUNODEFICIENCY VIRUS TYPE 1 GP120 USING HUMAN MONOCLONAL ANTIBODIES FROM PHAGE DISPLAY LIBRARIES
【24h】

MAPPING THE PROTEIN SURFACE OF HUMAN IMMUNODEFICIENCY VIRUS TYPE 1 GP120 USING HUMAN MONOCLONAL ANTIBODIES FROM PHAGE DISPLAY LIBRARIES

机译：使用来自噬菌体展示库的人类单克隆抗体来映射人免疫缺陷病毒1型GP120的蛋白质表面

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Panels of hybridoma-derived monoclonal antibodies against diverse epitopes are widely used in defining protein surface topography, particularly in the absence of crystal or NMR structural information. Here we show that recombinant monoclonal antibodies from phage display libraries provide a rapid alternative for surface epitope mapping. Diverse epitopes are accessed by presenting antigen to the library in different forms, such as sequential masking of epitopes with existing antibodies or ligands prior to selection and selection on peptides. The approach is illustrated for a recombinant form of the human immunodeficiency virus type 1 (HIV-1) surface glycoprotein gp120 which has been extensively mapped by rodent and human monoclonal antibodies derived by cellular methods. Human recombinant Fab fragments to most of the principal epitopes on gp120 are selected including Fabs to the C1 region, a C1/C5 epitope, a C1/C2 epitope, the V2 loop, the V3 loop and the CD4 binding domain. In addition an epitope linked to residues in the V2 loop and CD4 binding domain is identified. Most of these specificities are associated with epitopes presented poorly on native multimeric envelope, consistent with the notion that these antibodies are associated with immunization by forms of gp120 differing in conformation from that found on whole virus or infected cells. (C) 1997 Academic Press Limited. [References: 45]
机译：针对各种表位的杂交瘤来源的单克隆抗体组广泛用于定义蛋白质表面形貌，特别是在缺乏晶体或NMR结构信息的情况下。在这里，我们显示了来自噬菌体展示文库的重组单克隆抗体为表面表位作图提供了一种快速的替代方法。通过以不同形式将抗原呈递给文库来访问不同的表位，例如在选择和选择肽之前先用现有的抗体或配体对表位进行顺序掩盖。举例说明了重组形式的人类免疫缺陷病毒1型（HIV-1）表面糖蛋白gp120的方法，该方法已被通过细胞方法衍生的啮齿动物和人类单克隆抗体广泛定位。选择针对gp120上大多数主要表位的人重组Fab片段，包括针对C1区域，C1 / C5表位，C1 / C2表位，V2环，V3环和CD4结合域的Fab。另外，鉴定了与V2环和CD4结合结构域中的残基连接的表位。这些特异性中的大多数与在天然多聚体包膜上表现不佳的表位有关，这与以下观念有关：这些抗体与通过gp120形式进行的免疫接种相关，其形式不同于在完整病毒或感染细胞上发现的形式。 （C）1997 Academic Press Limited。 [参考：45]

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号