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Saccharomyces cerevisiae MSH2, a mispaired base recognition protein, also recognizes Holliday junctions in DNA

机译:酿酒酵母MSH2是一种配对错误的碱基识别蛋白,也可以识别DNA中的霍利迪连接

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Genetic and biochemical studies have suggested that mismatch repair proteins interact with recombination intermediates to prevent recombination, or to limit the extent of formation of heteroduplex DNA during recombination between divergent DNA sequences. To test the idea that mismatch repair proteins regulate recombination by interacting with recombination intermediates, we investigated whether the Saccharomyces cerevisiae MutS homolog MSH2 could interact with Holliday junctions. Both filter-binding and electron-microscopic analysis showed that MSH2 bound to duplex DNA molecules containing Holliday junctions with a higher affinity than to control duplex DNA, single-stranded DNA or a control duplex DNA containing a mispaired base. The MSH2-Holliday junction complexes were also more stable than MSH2-duplex DNA complexes. This observation suggests that MSH2 protein could directly coordinate the interaction between mismatch repair and genetic recombination observed in genetic studies.
机译:遗传和生化研究表明,错配修复蛋白与重组中间体发生相互作用,以防止重组,或限制异源DNA序列重组期间异源双链DNA的形成程度。为了测试错配修复蛋白通过与重组中间体相互作用来调节重组的想法,我们调查了酿酒酵母MutS同源MSH2是否可以与霍利迪接头相互作用。过滤器结合和电子显微镜分析均显示,MSH2以比亲和双链DNA,单链DNA或含有错配碱基的对照双链DNA更高的亲和力与含有霍利迪结的双链DNA分子结合。 MSH2-Holliday连接复合物也比MSH2-双链DNA复合物更稳定。该观察结果表明,MSH2蛋白可以直接协调基因研究中观察到的错配修复与基因重组之间的相互作用。

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