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首页> 外文期刊>Journal of Molecular Biology >Three-dimensional structure of bovine NADH : Ubiquinone oxidoreductase (Complex I) at 22 angstrom in ice
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Three-dimensional structure of bovine NADH : Ubiquinone oxidoreductase (Complex I) at 22 angstrom in ice

机译：牛NADH的三维结构：冰中22埃的泛醌氧化还原酶（复合体I）

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摘要

NADH:ubiquinone oxidoreductase (complex I) is the first and largest complex in the electron transport chain of mitochondria. The bovine complex purified from cardiac muscle consists of at least 42 different subunits with a combined molecular mass of about 890 kDa. The three-dimensional structure of the complex was determined at 22 Angstrom from single particles embedded in vitrified ice using electron cryo-microscopy. The structure was calculated using a new program to align particles, to correct for the contrast transfer function of the microscope, and to carry out the three-dimensional reconstruction of the complex. The bovine complex has the overall L-shaped appearance found in earlier studies of the closely related complex I from Neurospora crassa, but it differs by having a thin stalk region Linking the membrane-bound globular arm with the intrinsic membrane domain. Thus, the stalk which measures about 30 Angstrom in diameter is likely to contain part of the electron transfer pathway linking the NADH binding site in the globular arm with the ubiquinone binding site in the membrane domain. The globular domain of bovine complex I is significantly bigger than that of the N. crassa enzyme, suggesting that the apparent additional subunit complexity of the bovine enzyme is associated with the globular part. (C) 1998 Academic Press Limited. [References: 47]
机译：NADH：泛醌氧化还原酶（复合物I）是线粒体电子传输链中的第一个也是最大的复合物。从心肌中纯化的牛复合物由至少42个不同的亚基组成，其总分子量约为890 kDa。使用电子冷冻显微镜从嵌入玻璃化冰中的单个颗粒在22埃下确定复合物的三维结构。使用新程序计算结构，以对齐粒子，校正显微镜的对比度传递函数以及对复合物进行三维重建。牛复合物的整体形状呈L形，这是在早期对来自神经孢霉的复合物I的研究中发现的，但是它的区别在于其茎区域较薄，将膜结合的球状臂与固有的膜结构域相连。因此，直径约30埃的茎可能包含一部分电子转移途径，该途径将球状臂中的NADH结合位点与膜结构域中的泛醌结合位点连接起来。牛复合体I的球状结构域明显大于N. crassa酶的球形结构域，表明牛酶的表观其他亚基复杂性与球状部分有关。 （C）1998 Academic Press Limited。 [参考：47]

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