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首页> 外文期刊>Journal of Molecular Biology >Basis for monomer stabilization in Rhodopseudomonas palustris cytochrome c ' derived from the crystal structure
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Basis for monomer stabilization in Rhodopseudomonas palustris cytochrome c ' derived from the crystal structure

机译：来源于晶体结构的红假单胞菌细胞色素c'的单体稳定化基础

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The crystal structure of an unusual monomeric cytochrome c' from Rhodopseudomonas palustris (RPCP) has been determined at 2.3 Angstrom resolution. RPCP has the four-helix (helices A, B, C and D) bundle structure similar to dimeric cytochromes c'. Ho ct ever the amino acid composition of the surface of helices A and B in RPCP is remarkably different from that of the dimeric cytochromes c'. This surface forms the dimer interface in the latter proteins. RPCP has seven charged residues on this surface contrary to the dimeric cytochromes c', which have only two or three on the corresponding surface. Moreover, hydrophobic charged groups residues on this surface of RTCP are two to three times fewer than in dimeric cytochromes c'. As a result of the difference in amino acid composition, the A-B surface of RTCP is rather hydrophilic compared with dimeric cytochromes c'. We thus suggest that RPCP is monomeric in solution because of the hydrophilic nature of the A-B surface. The amino acid composition of the A-B surface is similar to that of Rhodobacter capsulatus cytochrome c' (RCCP), which is an equilibrium admixture of monomer and dimer. The charge distribution of the A-B surface in RCCP, however, is considerably different from that of RPCP. Due to the difference, RCCP can form dimers by both ionic and hydrophobic interactions. These dimers are quite different h-om those in proteins which form strong dimers such as in Chromatium vinosum, Rhodospirillum rubrum, Rhodospirillum molischianum and Alcaligenes. Cytochrome c' can be classified into two types. Type 1 cytochromes c' have hydrophobic A-B surfaces and they are globular. the A-B surface of type 2 cytochromes c' is hydrophilic and they take a monomeric or flattened dimeric form. (C) 1998 Academic Press. [References: 36]
机译：已经从2.3埃分辨率下确定了来自红假单胞菌（RPCP）的异常单体细胞色素c'的晶体结构。 RPCP具有类似于二聚体细胞色素c'的四螺旋（螺旋A，B，C和D）束结构。但是，RPCP中螺旋A和B表面的氨基酸组成与二聚体细胞色素c'的氨基酸组成显着不同。该表面在后面的蛋白质中形成二聚体界面。与二聚体细胞色素c'相反，RPCP在该表面上具有七个带电残基，后者在相应表面上仅具有两个或三个。此外，RTCP表面上的疏水性带电基团残基比二聚细胞色素c'中的残基少2至3倍。由于氨基酸组成的差异，与二聚体细胞色素c'相比，RTCP的A-B表面相当亲水。因此，由于A-B表面的亲水性，我们认为RPCP在溶液中是单体的。 A-B表面的氨基酸组成与荚膜红细菌细胞色素c'（RCCP）相似，后者是单体和二聚体的平衡混合物。但是，RCCP中A-B表面的电荷分布与RPCP有很大不同。由于存在差异，RCCP可以通过离子和疏水相互作用形成二聚体。这些二聚体与形成强二聚体的蛋白质中的二聚体截然不同，例如在Chromatium v​​inosum，Rhodospirillum rubrum，Rhodospirillumum molischianum和Alcaligenes中。细胞色素c'可以分为两种类型。 1型细胞色素c'具有疏水性A-B表面，并且呈球形。 2型细胞色素c'的A-B表面是亲水性的，呈单体或扁平二聚体形式。 （C）1998年学术出版社。 [参考：36]

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