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首页> 外文期刊>Journal of Molecular Biology >Folding intermediates of wild-type and mutants of barnase. II. Correlation of changes in equilibrium amide exchange kinetics with the population of the folding intermediate
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Folding intermediates of wild-type and mutants of barnase. II. Correlation of changes in equilibrium amide exchange kinetics with the population of the folding intermediate

机译：野生型和barnase突变体的折叠中间体。二。平衡酰胺交换动力学变化与折叠中间体的相关性

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There is an unanswered question from previous studies of H-1/H-2-exchange of amide protons of barnase. Under certain conditions, there is a relatively abrupt change from EX2 towards EX1 kinetics as the temperature is slightly increased. The change in kinetics for different mutants is not directly related to their changes in stability. We have measured the stability of the folding intermediate of barnase (I) in (H2O)-H-2 under a variety of conditions and calculated its population at different temperatures. The change in kinetics correlates with the change in the population of the folding intermediate. At higher temperatures and pH, the free energy of I becomes higher than that of the denatured state, D, and the kinetics becomes EX1. The data fit a simple kinetic scheme. Such changes in kinetics may be used to detect the presence of intermediates in the folding reaction at equilibrium in native conditions, but cannot distinguish whether they are on or off-pathway. (C) 1998 Academic Press Limited. [References: 33]
机译：从以前的研究中未发现有任何答案，即H-1 / H-2-交换芽孢杆菌酰胺酰胺质子。在某些条件下，随着温度的略微升高，从EX2到EX1动力学的变化相对突然。不同突变体的动力学变化与稳定性没有直接关系。我们已经测量了在各种条件下（H2O）-H-2中的Barnase（I）折叠中间体的稳定性，并计算了其在不同温度下的种群。动力学变化与折叠中间体的数量变化相关。在较高的温度和pH值下，I的自由能变得高于变性状态D的自由能，并且动力学变为EX1。数据符合简单的动力学方案。动力学的这种变化可用于检测在天然条件下处于平衡状态的折叠反应中中间体的存在，但不能区分中间体是在路径上还是在路径外。 （C）1998 Academic Press Limited。 [参考：33]

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