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首页> 外文期刊>Journal of Molecular Biology >Conformational analysis of the first observed non-proline cis-peptide bond occurring within the complementarity determining region (CDR) of an antibody
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Conformational analysis of the first observed non-proline cis-peptide bond occurring within the complementarity determining region (CDR) of an antibody

机译：构象分析的第一个观察到的非脯氨酸顺肽键出现在抗体的互补决定区（CDR）

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An analysis has been performed on the first example of a non-proline cis-peptide bond found within a complementarity determining region (CDR) of an antibody. The bond is located in CDR 3 of the heavy chain (H3) and makes substantial interactions to a peptide from a breast tumour-associated antigen. The antibody-peptide complex is compared, both in H3 length (six residues) and peptide conformation, to a number of other such complexes in the Brookhaven Data Bank (PDB). There is only one other H3 loop of the same length. Analysis of loop searches of the PDB, taken over the H3 framework of SM3, suggest that there is a limited repertoire of conformations for loops of length 6 compared to loops of length 5 and 7. It is argued that the cis-peptide bond is present because of the limited number of loop conformations of length 6, plus, the requirement of the H3 loop to contact the bound peptide. Modelling suggests that an all-trans-peptide loop conformation can replace the H3 loop and this raises the question of whether there is a trans- to cis-peptide bond isomerization upon peptide binding. (C) 1998 Academic Press. [References: 34]
机译：已经对在抗体的互补决定区（CDR）内发现的非脯氨酸顺肽键的第一个实例进行了分析。该键位于重链（H3）的CDR 3中，并与来自乳腺肿瘤相关抗原的肽发生实质性相互作用。在H3长度（六个残基）和肽构象上，将抗体-肽复合物与Brookhaven数据库（PDB）中的许多其他此类复合物进行了比较。只有另一个相同长度的H3回路。分析SM3的H3框架对PDB进行的环搜索分析表明，与长度5和7的环相比，长度6的环的构象库有限。有人认为存在顺式肽键由于长度为6的环构象的有限数目，加上H3环接触结合的肽的要求。建模表明，全反肽环构象可以取代H3环，这提出了在肽结合后是否存在反式至顺式肽键异构化的问题。 （C）1998年学术出版社。 [参考：34]

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